孤儿响应调节器CovR在从猪中分离的Streptococcus suis血清型2的生存能力和致病性方面发挥了积极的调节功能
Yanyan Zhang1, Rui Li1, Qian Li1
1Hubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Wuhan Polytechnic University, Wuhan, 430023, China.
BMC veterinary research
|November 22, 2023
概括
孤儿反应调节器CovR对于Streptococcus suis血清型2 (S. suis2) 的生存和致病性至关重要. 删除CovR削弱了S. suis 2,减少了它在小鼠中的生长,毒性和殖民.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 动物传播疾病 动物传播疾病
背景情况:
- 斯特雷波氏菌 suis 血清型 2 (S. suis 2) 是一个重要的动物传染病原体.
- 孤儿反应调节器CovR对于S. suis 2的生存和致病性至关重要.
- 关于CovR在S. suis 2中的具体作用的研究有限.
研究的目的:
- 调查S. suis 2中CovR的调控功能,生存能力和致病性.
- 分析CovR删除对S. suis 2毒性因子和宿主相互作用的影响.
主要方法:
- 产生了一种S. suis 2的CovR删除突变 (ΔcovR).
- 对比了野生型和ΔcovR菌株的生长速度,囊多糖体厚度,粘附性,入侵性和对细胞的抗性.
- 评估了野生型和 ΔcovR 菌株感染后的殖民能力和小鼠生存率.
- 分析了 ΔcovR 的代谢途径变化,使用了转录或蛋白质基因方法.
主要成果:
- 对CovR的删除显著影响了S. suis 2的生存能力和病原性.
- ΔcovR的生长减少,囊变薄,对宿主细胞的粘附/入侵减少.
- 在小鼠中,ΔcovR对细胞分裂和血清杀伤的抵抗性降低,并减少了小鼠的殖民.
- 对CovR的删除导致了关键代谢途径的显著下调,包括果糖/甘代谢和ABC载体.
结论:
- 在S. suis 2 SC19菌株中,CovR积极调节了生存能力和致病性.
- 对于S. suis 2的毒性,宿主殖民和代谢平衡来说,CovR是必不可少的.
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