细胞对由分裂酵母中的大气压等离子体诱导的复合应激的反应
Yoko Otsubo1, Akira Yamashita1,2, Yuhei Goto3,4,5
1Interdisciplinary Research Unit, National Institute for Basic Biology, Nishigonaka 38, Myodaiji, Okazaki, Aichi 444-8585, Japan.
Journal of cell science
|November 22, 2023
概括
被暴露在新型多重压力诱导剂等离子体辐射下的裂变酵母显示出抑制的生长. 研究人员确定了Sep1-Ace2细胞分离和TORC1通路是管理这些复杂的细胞应激的关键.
科学领域:
- 细胞生物学 细胞生物学
- 应激反应机制 应激反应机制
- 血医学是一种血医学.
背景情况:
- 对单个压力因素的细胞反应是众所周知的.
- 细胞对多个同时发生的压力因素的反应机制在很大程度上是未知的.
- 非热气压等离子体是一种新型的诱导器,能够产生多个同时的应力.
研究的目的:
- 描述裂变酵母细胞对非热气压等离子体的反应.
- 确定参与应对由等离子体辐射引起的多重压力的分子途径.
主要方法:
- 裂变酵母的直接等离子体辐射.
- 对裂变酵母生长抑制的分析.
- 对缺乏sep1和ace2转录因子的突变物进行基因组分析.
- 对Sep1-目标基因的转录分析.
- 针对拉帕素激酶复合体1 (TORC1) 的向抑制试验.
主要成果:
- 强烈的血辐射抑制了裂变酵母的生长.
- 缺乏sep1和ace2的突变体对等离子体辐射具有抗性.
- 轻度等离子体辐射降低了Sep1目标转录的调节.
- 等离子体辐射抑制了TORC1通路.
结论:
- 细胞分离路径Sep1-Ace2和TORC1路径对于裂变酵母来说至关重要,以应对由血辐射引起的多重压力.
- 这项研究阐明了由多重压力剂激活的新型细胞应激反应途径.
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