西斯普拉丁通过增加DEC1的减少介导的PXR来增加碳素度酶
Minqin Xu1, Lihua Zhang1, Lan Lin1
1Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
Journal of biomedical research
|November 22, 2023
概括
cis-Diamminedichloroplatinum (CDDP) 通过通过降低分化胚胎冠状细胞表达基因1 (DEC1) 上调孕妇X受体 (PXR) 来增加碳素酶1和2 (CES1/CES2). 这种机制增强了癌症治疗潜力.
科学领域:
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
- 肝病学 肝病学是一种肝病学.
背景情况:
- cis-Diamminedichloroplatinum (CDDP) 是固体瘤的基石化疗法之一.
- 了解CDDP的分子机制对于优化癌症治疗至关重要.
研究的目的:
- 阐明CDDP通过哪些分子途径影响碳素聚酶表达的分子途径.
- 调查孕妇X受体 (PXR) 和胚胎状细胞表达基因1 (DEC1) 在CDDP的影响中的作用.
主要方法:
- 评估了CDDP对人类肝瘤细胞和小鼠肝脏/肠道中CES1/CES2表达和活性的影响.
- 利用了PXR和DEC1.1的基因过度表达和淘汰技术.
- 进行了报告测试,以评估DEC1.1的转录调节.
主要成果:
- CDDP显著增加了CES1和CES2的表达和活动.
- 确定了PXR激活是CDDP诱导的炭基酶上调调节的关键媒介.
- 发现DEC1下调是PXR激活的上游,这表明了一个新的调节轴.
- CDDP通过转录抑制了DEC1.
- 结合CDDP和义诺他干显示出协同效应,特别是当CDDP第一次被管理时.
结论:
- 通过一种涉及PXR激活和DEC1下调的途径,CDDP增强了碳素乙酶的表达.
- 这项研究揭示了CDDP作用的新机制,对组合疗法有影响.
- 准DEC1-PXR-CES轴可能为癌症治疗提供新的策略.
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