CDK5R1,GSE1,HSPG2和WDFY3作为在心房的间接表观遗传敏感基因
Teresa Infante1, Mark E Pepin2,3, Antonio Ruocco4
1Department of Advanced Medical and Surgical Sciences (DAMSS), University of Campania "Luigi Vanvitelli", Naples, Italy.
European journal of clinical investigation
|November 22, 2023
概括
血液中CD4+T细胞的表观遗传变化显示出诊断心房动 (AF) 的前景. 像CDK5R1和HSPG2这样的基因中的DNA甲基化模式可以帮助非侵入性地识别AF患者.
科学领域:
- 心血管表观遗传学 心血管表观遗传学
- 分子诊断学 分子诊断
- 精准医学是一门精准的医学.
背景情况:
- 异常的DNA甲基化与心房动 (AF) 病变发生有关.
- 目前缺乏用于AF诊断的非侵入性表观遗传生物标志物.
研究的目的:
- 为了研究AF患者外周血液CD4+T细胞中的DNA甲基组变化.
- 为了确定潜在的非侵入性表观遗传生物标志物用于AF诊断.
主要方法:
- 减少代表性双硫酸盐测序 (RRBS) 用于分析CD4+T细胞中的DNA甲基化.
- 从10名AF患者和11名健康受试者 (HS) 收集了外周血液样本.
- 对甲基化和基因表达数据进行了生物信息分析,包括网络构造和ROC曲线分析.
主要成果:
- 一个心房特异性网络确定了18个差异甲基化基因 (DMG).
- 像CDK5R1,HSPG2,WDFY3和COL4A1这样的基因表现出显著的诊断性能 (AUC从0.76到0.90不等).
- 与HS患者相比,AF患者观察到包括CDK5R1,HSPG2,WDFY3和GSE1在内的基因过度表达.
结论:
- 根据甲基化和表达水平,CDK5R1,GSE1,HSPG2和WDFY3成为了最高的歧视基因.
- 这些发现表明AF的候选诊断生物标志物.
- 已识别的生物标志物有可能在AF管理中推进精准医学方法.
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