波伦戈奶酪乳清:一种新基质,用于生产β-银酸酶
Rafaela J S Coelho1, Sabrina Gabardo2, Aline Vitória C Marim2
1Federal University of São Carlos, Center of Natural Sciences, Rodovia Lauri Simões de Barros, Km 12, 18245-970 Buri, SP, Brazil.
Anais da Academia Brasileira de Ciencias
|November 22, 2023
概括
这项研究证明了使用Kluyveromyces marxianus的Porungo奶酪乳清有效生物转化为β-galactosidase. 使用K. marxianus CCT 4086,酵母提取物补充剂,pH值7.0和30°C实现了最佳的酶生产.
科学领域:
- 生物技术和工业微生物学
- 酶的生产 酶的生产
- 废弃物价值化 废弃物价值化 废弃物价值化
背景情况:
- 作为乳制品加工的副产品,Porungo奶酪乳清通常被不足利用.
- β-银酸酶是一种有价值的酶,在食品工业中具有应用,特别是乳糖水解.
- 微生物发酵为酶生产提供了一个可持续的途径.
研究的目的:
- 为了研究波伦戈奶酪乳清的生物转化,以生产β-galactosidase.
- 为了评估两个Kluyveromyces marxianus菌株 (CCT 4086和CBS 6556) 的性能.
- 为了确定最佳的发酵条件 (温度,pH,源) 以最大限度地提高酶产量.
主要方法:
- 使用Kluyveromyces marxianus菌株进行Porungo奶酪乳清的批量发酵.
- 用不同的源补充乳清,包括酵母提取物.
- 优化发酵参数:温度 (30°C和37°C) 和pH (5.07.0).
- 酶活性测定以量化β-galactosidase的产量.
主要成果:
- 与CBS 6556.6相比,Kluyveromyces marxianus CCT 4086表现出比较高的β-银酸酶产生.
- 最高的酶活性 (16.73 U mL−1) 是通过K. marxianus CCT 4086在乳清中补充酵母提取物而获得的.
- β-银酸酶最大活性的最佳条件是pH 7.0和30°C.
- 在所有测试的pH值中,在37°C时观察到较低的酶活性.
结论:
- 波伦戈奶酪乳清是工业β-银酸酶生产的可行和成本有效的基质.
- 克劳维罗米塞斯马克西南斯 (Kluyveromyces marxianus) CCT 4086是一种有前途的菌株,可以有效地生物合成β-银酸酶.
- 优化的发酵条件显著提高了酶产量,突出了可持续生物工艺的潜力.
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