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从体积微样本中对六种抗生素进行生物分析:一种用于在重症儿童中精确剂量的新工具

John Takyi-Williams¹, Abbie D Leino¹, Ruiting Li¹

¹College of Pharmacy, University of Michigan, Ann Arbor, MI 48108, USA.

|November 22, 2023

概括

容量吸收微样 (VAMS) 允许在药理动力学研究中同时量化多种抗生素. 这种方法支持药物监测和治疗疗效评估.

科学领域:

分析化学分析化学
药理动力学药理动力学
生物分析是一种生物分析.

背景情况:

容量吸收微样 (VAMS) 为收集生物样本提供了一种最少的侵入性方法.
药理动力学和药理动力学 (PK/PD) 研究对于了解药物行为和疗效至关重要.
对于复杂的治疗方案,同时量化多种抗生素是必不可少的.

研究的目的:

开发和验证一种生物分析方法,用于在VAMS中量化七种抗生素 (安皮西林,塞菲皮姆,塞夫triaxone,meropenem, piperacillin,tazobactam,vancomycin).
评估这些抗生素在VAMS中的稳定性.
为了评估潜在的干扰,如血红素效应.

主要方法:

优化提取条件,使用水进行再水和甲醇进行蛋白质沉.
开发一种经过验证的液体染色学-并联质谱法 (LC-MS/MS) 方法.
使用了Phenomenex Kinetex Polar C18柱子,其中含有酸的水/氨移动相.

主要成果:

目标抗生素的提取恢复率很高.
这种LC-MS/MS方法证明了完全的验证.
血红素干扰仅观察到万科米辛.
在VAMS中,抗生素的稳定性在-80°C下被证实长达90天 (美罗:60天).

结论:

成功开发了一种强大的生物分析方法,用于在VAMS中同时量化多种抗生素.
这种方法可以通过单个微样本对多种药物的有效分析来促进PK/PD研究.
这些发现支持使用VAMS用于治疗药物监测和优化抗生素治疗.

关键词:

贝塔乳糖类药物贝塔乳糖类干燥的血液斑点干燥的血液斑点葡萄糖类是葡萄糖类中的一种.传染病是一种传染性疾病.治疗药物监测治疗药物监测容量微量采样容量微量采样

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