在人类ES细胞中,YTHDF1促进了PRC1介导的H2AK119ub
Jingyuan Zhang1,2,3,4,5,6, Tianyu Wang1,2,3,4,5, Ruona Shi2,3
1CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Journal of cellular physiology
|November 22, 2023
概括
YTHDF1,一种RNA结合蛋白,最近在人类胚胎干细胞的核中发现,它与PRC1相互作用,调节基因表达和神经发育.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 多胞体抑制复合体 (PRCs) 对于细胞命运决定至关重要,并与疾病有关.
- PRCs通过像H3K27me3和H2AK119ub.这样的基因组修饰进行中介.
- 对于PRC对染色质的招募机制尚不完全理解.
研究的目的:
- 为了研究YTHDF1在Polycomb抑制复合体1 (PRC1) 功能中的作用.
- 在人类胚胎干细胞 (hESCs) 中探索YTHDF1的非正规核功能.
主要方法:
- 同免疫沉以评估蛋白质相互作用.
- 染色体免疫沉用于识别基因标.
- 基因淘汰的RNA干扰 (RNAi).
- 免疫光检测用于确定蛋白质定位.
主要成果:
- 发现YTHDF1,通常是细胞质的,在hESCs的核中定位.
- 核YTHDF1与PRC1组成部分RNF2以及H2AK119ub.相关.
- 抑制YTHDF1降低了H2AK119ub水平,并促进了神经分化.
结论:
- YTHDF1在核内PRC1介导的基因调节中发挥着非正规的作用.
- YTHDF1影响H2AK119ub修饰和hESC中的神经分化.
- 这项研究揭示了一种新的机制,将RNA结合蛋白与表观遗传调节联系起来.
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