对转录组进行比较分析,揭示了与 Schisandra chinensis 的性别分化相关的候选基因
Ji-Long Cheng1, Xue-Ping Wei2,3, Yu Chen1
1Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Functional & integrative genomics
|November 22, 2023
概括
这项研究揭示了控制 Schisandra chinensis 花朵发育的关键基因,这对其宝贵的水果产量至关重要. 了解性别差异化机制可以帮助调节男性和女性花的比例.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生殖生物学 生殖生物学
背景情况:
- 奇桑德拉中华果具有重要的药用和经济价值.
- 果实产量受到雌雄花的比例的影响.
- 有限的研究存在于S. chinensis的花发育和性别差异化.
研究的目的:
- 为了研究基底的分子机制的性别差异化在schisandra chinensis.
- 识别参与花性别决定的差异表达基因 (DEGs).
- 提出一种监管模型,用于该物种的性别差异化.
主要方法:
- 在三个发育阶段对雌雄花的RNA测序 (RNA-seq) 分析进行比较.
- 差异表达基因 (DEGs) 的识别和分析.
- 对MADS-box和YABBY基因家族进行KEGG通路分析和鉴定.
- 使用RT-qPCR验证RNA-seq结果.
主要成果:
- 在不同阶段的雌雄花之间确定了936,7179和6890个DEG.
- 466个候选基因与性别差异化有关.
- 黄类生物合成和DNA复制途径对于雌性花的发育至关重要.
- MADS-box和YABBY基因与性别决定密切相关.
- 通过RT-qPCR确认了20个DEG,其中17个偏向女性,3个偏向男性.
结论:
- 在Schisandra chinensis的性别差异化中发现了潜在的分子参与者.
- 突出了类生物合成,DNA复制,MADS-box和YABBY基因的作用.
- 为未来的研究奠定了基础,研究如何调节两性比率以提高水果产量.
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