相关实验视频

Updated: Jul 10, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

对于生产多基酸盐的Bacillus sp.的新组装和比较基因组分析. 这是BNPI-92菌株

Seid Mohammed Ebu¹, Lopamudra Ray², Ananta N Panda³

¹Department of Applied Biology, SoANS, Adama Science and Technology University, Oromia, Ethiopia. seid.mohammed@astu.edu.et.

Journal, genetic engineering & biotechnology

|November 22, 2023

概括

这项研究确定了Bacillus cereus BNPI-92作为一种新型菌株,具有聚酸酸盐 (PHA) 生物合成的潜力. 基因组分析证实了它的身份,并确定了关键的PHA生产基因.

科学领域:

微生物学微生物学
基因组学就是基因组学.
生物技术是生物技术.

背景情况:

细菌物种对于聚氨酸 (PHA) 生产至关重要.
在科学文献中,经常缺乏准确的物种级别识别PHA产生细菌分离物.

研究的目的:

对一种新型细菌分离物进行全面的基因组分析,该分离物被指定为BNPI-92.2.
为了确认Bacillus sp.的物种身份. BNPI-92使用比较基因组学.
为了研究这种菌株中聚酸酸盐 (PHA) 生物合成的遗传基础.

主要方法:

下一代测序 (NGS) 用于新的基因组组装.
用于基因组注释的RAST服务器.
用于对比基因组分析的OrthoVenn.
平均核酸标识 (ANI),in silico DNA-DNA杂交 (isDDH),TYGS和GGDC用于遗传学和分类学评估.

主要成果:

细菌菌种的基因组. 组装了BNPI-92,其中包含5719个预测基因.
包括OrthoVenn,ANI和isDDH在内的比较基因组分析强烈表明,Bacillus sp. BNPI-92是一种Bacillus cereus的菌株.
参与PHA生物合成的关键基因 (phaP,phaQ,phaR,phaB/phbB,phaC) 在phaPQRBC操作子中被确定,phaA分别位于其中.

结论:

巴西勒斯种类 (Bacillus sp.) 的存在证实BNPI-92是一种新的Bacillus cereus菌株.
鉴定出来的菌株B. cereus BNPI-92,由于存在相关基因,它是聚酸酸盐 (PHA) 生物合成的有希望的候选者.

关键词:

一年多的时间.细菌种类的细菌物种.基因基因基因基因基因基因组 DNA DNA 的基因组.代谢途径的代谢途径

更多相关视频

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Published on: May 16, 2017

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.