糖核糖治疗触发了与防御相关的反应,并改变了细胞壁的组成
Bhagwat Prasad Dewangan1, Arunima Gupta1, Rajan Kumar Sah1
1Laboratory of Plant Cell Wall Biology, Regional Centre for Biotechnology, NCR Biotech Science Cluster 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad, Haryana, 121001, India.
Plant molecular biology
|November 22, 2023
概括
短的西-寡糖 (XOS) 在植物中充当损伤相关分子模式 (DAMP),触发免疫反应并改变细胞壁组成. 这项研究表明XOS是XOS.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物免疫力 植物免疫力
- 植物细胞壁生物学 植物细胞壁生物学
背景情况:
- 植物细胞壁在发育或病原体攻击期间产生与损伤相关的分子模式 (DAMPs).
- 这些DAMP被植物细胞壁受体感知,调节免疫力和细胞壁结构.
- 假设西-寡糖化物 (XOS) 作为植物免疫反应的诱导者.
研究的目的:
- 调查一下,短XOS的xylobiose (XB) 是否会在Arabidopsis中引起植物免疫反应.
- 分析XB对植物免疫力,细胞壁组成和激素水平的影响.
- 为了比较单独对XB和与已知的微生物相关分子模式 (MAMP) 结合的分子和细胞反应.
主要方法:
- 阿拉比多普西斯植物被用西洛比 (XB) 处理.
- 测量包括反应性氧物种 (ROS) 生成,MAPK酸化,质沉积和化酶活性.
- 评估了基因表达分析 (RNA测序) 和细胞壁糖成分.
- 测量了荷尔蒙水平 (雅斯蒙酸,酸,辅酶,细胞因素).
主要成果:
- XB治疗显著诱导了ROS的产生,MAPK的激活和质沉积,表明植物免疫的激活.
- 结合XB和flg22 (MAMP) 治疗显示ROS和PTI标记基因表达的增强.
- RNA测序揭示了XB治疗后的快速和广泛的基因调节变化,与ROS反应相关.
- 细胞壁的组成发生了变化,增加了总糖和特定单糖水平的变化,同时改变了荷尔蒙特征.
结论:
- 在植物中,最短的西-寡糖 (XOS) 作为损伤相关分子模式 (DAMPs) 起作用.
- 治疗XB有效地触发了PTI (PAMP触发免疫) 反应.
- XOS感知会影响植物细胞壁组成和内源性激素水平,这表明植物信号传递中的复杂作用.
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