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通过同步单分子光和单通道电气记录,通过纳米孔确认静音转移

Daniel L Burden¹, Joshua J Meyer¹, Richard D Michael¹

¹Chemistry Department, Wheaton College, Wheaton, Illinois 60187, United States.

Analytical chemistry

|November 22, 2023

概括

研究人员发现了一种新现象,称为"静音转位",其中分子通过纳米孔而不产生电信号. 这需要先进的光技术来检测,为研究纳米孔运输开辟新的途径.

科学领域:

纳米孔科学与技术纳米孔科学与技术
单分子生物物理学单分子生物物理学
分子运输现象分子运输现象

背景情况:

目前对纳米孔传输的理解在很大程度上依赖于对离子电流的电测量.
纳米孔特征通常涉及检测与材料通道相关的电信号.
没有电 signature 的运输现象,称为"静音转移",仍然在很大程度上未被探索.

研究的目的:

通过纳米孔研究和描述静音转位.
用一种特定的光染料来证明静音转位的发生.
探索影响静音转移事件的因素,并量化运输参数.

主要方法:

在转位研究中使用了5 (sCy5a) 的衍生物.
采用同时进行单分子光和单通道电气记录.
在一个封闭的微腔层上形成了二层层,以方便纳米孔测量.

主要成果:

证实了sCy5a通过α-hemolysin (αHL) 纳米孔的罕见静音转位的发生.
观察到静音转位事件作为时间,染料度和纳米孔群的函数.
量化转移速率,有效的能量屏障和有效的染料充电,没有电 signature.

结论:

静音转位是分子通过纳米孔的可行现象.
辅助测量技术,如光,对于检测静音转位是必不可少的.
这项研究为探索纳米孔中以前隐藏的运输机制提供了基础.

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