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相关概念视频

Meiosis II02:02

Meiosis II

45.8K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
45.8K
Meiosis vs. Mitosis02:57

Meiosis vs. Mitosis

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Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
56.6K
Oogenesis01:22

Oogenesis

1.6K
Oogenesis,  the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
1.6K

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相关实验视频

Updated: Jul 10, 2025

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

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NUSAP1调节了小鼠卵细胞中介成熟的过程.

Lina Yu1,2, Na Kong1,3, Yuling Lin2

  • 1Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, China.

Journal of cellular biochemistry
|November 22, 2023
PubMed
概括

核细胞和关联蛋白1 (NUSAP1) 对于小鼠卵细胞化至关重要. 它的耗尽导致染色体错位和异常的螺旋组装,突出其在确保高质量的卵子细胞成功受精方面的作用.

关键词:
我们的NUSAP1介质变化 (meiosis) 是一种变质的过程.卵子细胞 卵子细胞生殖生殖的繁殖.螺旋的螺旋是一个螺旋.

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Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

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相关实验视频

Last Updated: Jul 10, 2025

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Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
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Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
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科学领域:

  • 细胞生物学 细胞生物学
  • 发展生物学 发展生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 精确的染色体分离在小鼠卵细胞化过程中对于卵细胞质量和受精至关重要.
  • 与关联的蛋白质调节了动力学,但NUSAP1在卵细胞化中的作用尚不清楚.
  • NUSAP1的表达显著增加了中转后的恢复,这表明它发挥了关键作用.

研究的目的:

  • 为了研究NUSAP1在小鼠卵细胞半结成熟过程中的作用.
  • 确定NUSAP1局部化及其对线组装和染色体分离的影响.

主要方法:

  • 蛋白质组分析以确定差异表达的关联蛋白质.
  • 免疫光显微镜用于跟踪卵细胞成熟期间的NUSAP1定位.
  • 通过基因编辑或RNA干扰减少NUSAP1.
  • 染色体扩散分析和形态评估.
  • 对NUSAP1互动组的RNA测序 (RNA-seq) 和基因本体学 (GO) 分析.

主要成果:

  • 在胚芽囊泡 (GV) 卵细胞中,NUSAP1定位在细胞核中,并在介质化I过程中在螺旋杆附近形成聚合物.
  • 由于NUSAP1的耗尽,导致染色体不对齐,化,并减少了杆杆宽度.
  • RNA-seq揭示了NUSAP1缺陷卵细胞中"建立螺旋方向"途径的抑制.
  • 对于RNA结合而言,NUSAP1互动体被丰富,这表明它在mRNA稳态和P体动力学中发挥了作用.

结论:

  • 在小鼠卵细胞中,NUSAP1对于正确的线组装和染色体分离至关重要.
  • 精确调节NUSAP1的表达和局部化对于保持介质忠实性至关重要.
  • NUSAP1可能通过RNA结合活动和对P体组件的影响来调节卵细胞半转化.