检测宿主对微生物刺激的反应,使用初级上皮器官有机体.
Jette Bornholdt1,2,3, Christina V Müller1, Maria Juul Nielsen1
1Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark.
Gut microbes
|November 22, 2023
概括
健康的肠道有机体对益生菌细菌表现出不同的反应,与癌症细胞系不同. 这项研究强调有机体是研究宿主微生物相互作用的更好的模型.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 细胞生物学 细胞生物学
背景情况:
- 肠道上皮与光线微生物相互作用.
- 癌症衍生细胞系 (例如,Caco-2) 传统上用于研究这些相互作用.
- 目前尚不清楚这些细胞系是否准确地代表了正常的上皮反应和菌株特异性的微生物影响.
研究的目的:
- 通过使用有机体来研究正常肠道上皮质中的微生物反应.
- 为了比较健康的有机体和Caco-2细胞系之间的反应.
- 为了确定常见和菌株特定的微生物相互作用.
主要方法:
- 来自健康个体的肠道器官 (n=9).
- 在拉米因基质上培养有机体,以诱导分化和尖端暴露.
- 暴露于益生菌细菌菌株的分化有机体 (BB-12®,LGG®,DSM33361,Bif195).
- 使用Caco-2细胞系进行并行实验.
主要成果:
- 健康的肠道器官对各种益生菌菌株表现出强大的急性反应.
- 在个体中观察到既有常见的,也有针对菌株的反应.
- 可可-2细胞系对测试中的益生菌没有表现出急性反应.
- 机体模型揭示了微生物反应的个体变化.
结论:
- 来自健康个体的初级肠道器官是研究宿主-微生物相互作用的有价值模型.
- 与Caco-2细胞相比,有机体提供了一个更准确的表现正常的上皮反应.
- 这种方法提高了对宿主微生物动态和微生物菌株特异性的个体变异的理解.
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