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Updated: Jul 10, 2025

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一个染色体规模的基因组的Peucedanum praeruptorum提供了关于Apioideae进化和药物成分生物合成的见解

Cheng Song¹, Yingyu Zhang², Muhammad Aamir Manzoor³

¹Anhui Dabieshan Academy of Traditional Chinese Medicine, Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Anhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine, College of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China.

International journal of biological macromolecules

|November 22, 2023

概括

实现了药用植物Peucedanum praeruptorum的高质量基因组组. 这种基因组揭示了全基因组重复事件和扩展的基因家族,这些基因家族对于氨酸和类生物合成至关重要.

科学领域:

基因组学就是基因组学.
植物科学植物科学
药用化学医学化学

背景情况:

唐 (Peucedanum praeruptorum Dunn) 是一种富含氨酸的中国传统医药.
缺乏高质量的基因组阻碍了对氨酸生产基因的研究.
了解其基因组对于药用植物和芳香植物研究至关重要.

研究的目的:

测序并组装P. praeruptorum的第一个染色体尺度参考基因组.
为了研究氨酸和类生物合成的基因组基础.
为了探索阿皮叶科家族的进化历史.

主要方法:

牛津纳米孔技术和Hi-C测序用于基因组组装.
比较基因组学以确定基因组和基因家族扩展.
全基因组复制 (WGD) 和三倍化 (WGT) 事件的分析.

主要成果:

一个1.83Gb的染色体规模的基因组被组装在一起,具有高度的连续性和完整性.
确定了两个WGD事件和一个WGT事件,其中特定的WGD仅适用于Apials和Apiaceae.
观察到细胞染色体P450,O-甲基转移酶,ABC载体和合成酶等基因家族的扩张,与氨酸/氨酸生产有关.

结论:

在P. praeruptorum基因组为研究氨酸生物合成提供了宝贵的资源.
整个基因组复制事件在Apioideae.的氨酸和烯通路的演变中发挥了重要作用.
扩展的基因家族,特别是UDP-glucosyltransferases和ABC载体,可能参与氨酸的修饰和运输.

关键词:

亚科 (Apiaceae) 是一个亚科.库马林生物合成丹恩 (Dunn) 的皮塞丹姆 (Peucedanum praeruptorum) 是一个非常古老的植物.类生物合成类生物合成整个基因组的重复.

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