适应寒冷的和与之密切相关的科植物种类殖民于干燥老化的牛肉 (DAB)
Grzegorz Ostrowski1, Danuta Jaworska2, Magdalena Płecha3
1Institute of Evolutionary Biology, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089, Warsaw, Poland.
Fungal biology
|November 22, 2023
概括
干熟牛肉 (DAB) 的味道受到表面真菌的影响. 这项研究使用培养和DNA方法确定了主要的真菌物种,包括Mucor flavus,从而增强了我们对DAB微生物学的理解.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 干熟牛肉 (DAB) 由于其独特的味道而受到重视,历史上归因于肉表面的微生物活动.
- 之前的研究已经确定了Thamnidium和Mucor属的真菌是DAB的关键殖民者.
- 了解特定的真菌群落对于控制和优化DAB生产至关重要.
研究的目的:
- 调查干老牛肉 (DAB) 上的真菌群体组成.
- 具体识别DAB.上存在的粘菌类 (来自Mucorales序列的真菌) 种类.
- 为了比较基于培养的方法与DNA元编码的结果.
主要方法:
- 使用传统的基于培养的技术来隔离和识别真菌.
- 内部转录间隔器2 (ITS2) rDNA元编码分析用于全面的真菌群落概况.
- 通过培养和metabarcoding方法获得的数据的比较分析.
主要成果:
- 从Mucoraceae家族中分离出六种真菌物种,包括Mucor和Helicostylum属.
- 转基因编码证实了其他真菌的存在,例如Thamnidium属的真菌.
- 这两种方法都显示,与Mucor flavus菌株CBS 992.68密切相关的分离物占主导地位.
结论:
- 在DAB上的真菌群落是多样化的,包括来自Mucoraceae家族的物种.
- 黄 (Mucor flavus) 似乎是一个与DAB相关的重要真菌物种.
- 结合基于培养和metabarcoding的方法提供了对DAB真菌生态的强有力的理解.
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