通过对circFoxp1的上调调节,通过促进细胞增殖来影响 keloid
Jing Zhang1, Qianyi Bao2,3, Nan Song1
1Department of Facial Plastic and Reconstructive Surgery, ENT Institute, Eye and ENT Hospital, Fudan University, Shanghai, China.
Aging
|November 22, 2023
概括
像circFoxp1这样的循环RNA (circRNAs) 在 keloid 组织中升高,促进纤维细胞增殖和细胞外基质沉积. 这项研究确定circFoxp1作为潜在的治疗痕治疗的治疗标.
科学领域:
- 皮肤病学 皮肤病学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 质体是由于异常的伤口愈合而产生的,其特点是纤维细胞增殖和过度的细胞外基质 (ECM) 沉积.
- 目前治疗 keloids 的疗法提供有限的疗效.
- 圆形RNAs (circRNAs) 在 keloid 病原发生中的作用在很大程度上是未知的.
研究的目的:
- 为了研究circFoxp1在 keloid 形成中的功能.
- 为了探索circFoxp1作为潜在的治疗化体的目标.
主要方法:
- 定量逆转录-聚合酶连锁反应 (qRT-PCR) 来评估circFoxp1的表达.
- RNA-光in situ杂交 (RNA-FISH) 和RNAscope用于确定circFoxp1的局部化.
- 细胞实验评估circFoxp1过度表达对纤维细胞行为的影响.
- 用RNA测序来分析受circFoxp1.1.影响的细胞通路.
- 状体植入模型和体内研究.
- RNA pull-down和西部斑点测试以确定相互作用的蛋白质.
主要成果:
- 与正常组织相比,状组织中的circFoxp1表达显著更高.
- circFoxp1位于 keloid 纤维细胞的细胞质中.
- 过度表达circFoxp1增强了纤维细胞的增殖,迁移和ECM沉积,同时抑制了细胞亡.
- RNA测序显示circFoxp1影响纤维细胞中与炎症和氧化酸化相关的途径.
- 在体内研究证实,circFoxp1促进纤维细胞增殖和ECM沉积.
- 发现 circFoxp1 与 RACK1.1 相互作用.
结论:
- circFoxp1在 keloid 组织的病理性增生中发挥着重要作用.
- circFoxp1可以作为一种新的治疗点,用于管理 keloid 痕.
- 这项研究提供了新的洞察力,揭示了 keloids 的病原和潜在的治疗策略.
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