横向获取DNA结合酶可以提高真核生物的DNA损伤耐受性
Emilien Nicolas1, Paul Simion2,3, Marc Guérineau4
1Université Libre de Bruxelles, Molecular Biology and Evolution, Brussels, 1050, Belgium. emilien.nicolas@gmail.com.
Nature communications
|November 22, 2023
概括
布德洛伊德鱼具有独特的DNA连接酶E,通过横向基因转移获得,使其能够极端耐辐射. 这种酶增强了DNA修复,甚至在人体细胞中改善了放射性耐受性.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 进化生物学 进化生物学
背景情况:
- 贝德洛伊德虫对基因毒性压力表现出了显著的抵抗力.
- 了解这种极端耐受性背后的分子机制至关重要.
研究的目的:
- 为了研究bdelloid rotifer Adineta vaga极端辐射耐受性的分子基础.
- 确定有助于抗压力的新型DNA修复机制.
主要方法:
- 对暴露于电离辐射的Adineta vaga进行蛋白质组分析.
- 遗传学分析以确定已识别的DNA结合酶的起源.
- 功能性检测包括人类细胞系中的异质表达.
主要成果:
- 发现一种特定的DNA结合酶 (DNA结合酶E),与典型的真核生物修复酶不同,在暴露于辐射后过度表现.
- 遗传学分析表明,这种酶的水平基因从 prokaryotes 转移到 bdelloid rotifers.
- 菌Mortierella verticillata在暴露于X射线后也表现出增加的辐射耐受性和DNA酶E过度表达.
- 阿迪内塔瓦加结合酶E显示出显著的DNA断裂结合活性,对DNA修复途径至关重要.
- 人类细胞中Adineta vaga ligase E的异质表达增强了它们的放射性耐受性.
结论:
- 横向基因转移可以为真核生物提供适应性优势,例如极端耐压力.
- 基因链酶E在DNA修复和辐射耐受性方面发挥着重要作用.
- 这项研究强调了一种通过基因转移适应极端环境条件的新机制.
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