在乳腺癌中 lncRNA NORAD 的诊断影响
Yaping Zhang1, Xiaowei Fan2, Jianfeng Hong1
1Affliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, 311201, China.
Scientific reports
|November 22, 2023
概括
由DNA损伤激活的非编码RNA (NORAD) 在乳腺癌 (BRCA) 患者血清中升高. 这一发现表明,NORAD可以作为诊断乳腺癌的潜在生物标志物.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 非编码RNAs (ncRNAs) 在各种生物过程中起着至关重要的作用,包括癌症的发展.
- 长非编码RNAs (lncRNAs) 越来越多地被认为与瘤发生和进展有关.
- 由DNA损伤激活的非编码RNA (NORAD) 是一种涉及细胞功能和疾病状态的lncRNA.
研究的目的:
- 为了评估乳腺癌 (BRCA) 患者的NORAD表达水平.
- 调查NORAD与BRCA中的临床病理特征之间的关联.
- 探索NORAD作为乳腺癌诊断生物标志物的潜力.
主要方法:
- 在患者血清中使用定量逆转录PCR (RT-qPCR) 分析NORAD表达.
- 使用TCGA-BRCA,GSE77308,Cellminer和Sangerbox数据库进行全面的分析.
- 与临床病理学特征相关的NORAD表达,如TNM阶段和淋巴结转移.
主要成果:
- 在BRCA患者血清中,NORAD的表达明显高于健康个体和有良性乳腺结节的人.
- 升高的NORAD水平与BRCA患者的晚期TNM阶段和淋巴结转移相关.
- 诺拉德表达还显示与乳腺癌的光线分类有关.
结论:
- 在乳腺癌患者的血清中,NORAD的高调.
- 血清NORAD水平与BRCA的关键临床病理特征相关.
- 作为一个潜在的非侵入性诊断生物标志物和乳腺癌的治疗点,NORAD显示出前景.
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