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来自乌干达的SARS-CoV-2的基因组特征使用MinION纳米孔测序

Praiscillia Kia¹, Eric Katagirya², Fredrick Elishama Kakembo³

¹Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda. kiapriscilla@gmail.com.

Scientific reports

|November 22, 2023

概括

在乌干达样本中分析了导致COVID-19的病毒SARS-CoV-2的频繁突变. 调查结果表明本地传播,而不是新的进口,突出区域传播动态.

科学领域:

病毒学病毒学
基因组学就是基因组学.
流行病学流行病学

背景情况:

严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 经常发生突变,影响诊断准确性,疾病传播和疫苗有效性.
了解SARS-CoV-2的遗传多样性对于公共卫生干预至关重要.
之前的研究已经记录了乌干达和刚果民主共和国 (DRC) 的SARS-CoV-2菌株.

研究的目的:

来自乌干达的SARS-CoV-2样本的遗传多样性的特征,这些样本是在2020/2021年COVID-19浪潮期间收集的.
为了确定乌干达人口中普遍存在的SARS-CoV-2血统和突变.
推断乌干达SARS-CoV-2感染的起源,并评估区域传播模式.

主要方法:

来自乌干达的49个SARS-CoV-2样本的全基因组测序.
遗传学分析以确定病毒系.
单核酸变异 (SNVs) 和结构变异 (SVs) 的识别和表征.

主要成果:

大多数乌干达的SARS-CoV-2样本属于AY.46和A.23系,被确定为三角洲变种.
确定了大量的独特SNV (268) 和突变 (1456),主要是在ORF1ab和S基因中.
常见的突变包括2042C>G,14143C>T,245T>C和1129G>T. 还观察到结构变异 (21个插入,7个删除).
乌干达和刚果民主共和国变种之间的遗传相似性表明有显著的跨境传播.

结论:

在2020/2021年期间乌干达的SARS-CoV-2遗传概况表明,感染可能源于当地传播事件.
来自乌干达和刚果民主共和国的变种之间的密切遗传关系强调了该地区人类高度流动和相互作用的影响.
持续的基因组监测对于监测SARS-CoV-2演变和为区域公共卫生战略提供信息至关重要.

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