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Updated: Jul 10, 2025

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
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评估染色正常化技术在侵袭性管道癌的自动分级中的有效性 组织病理学图像

Wingates Voon1, Yan Chai Hum2, Yee Kai Tee1

  • 1Department of Mechatronics and Biomedical Engineering, Faculty of Engineering and Science, Lee Kong Chian, Universiti Tunku Abdul Rahman, Kampar, Malaysia.

Scientific reports
|November 22, 2023
PubMed
概括

斑点规范化 (SN) 对使用卷积神经网络 (CNN) 的侵袭性导管癌 (IDC) 分级准确度没有显著影响. 该研究发现,对于乳腺癌分类的正常化和非正常化数据集之间的差异很小.

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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