在眼科医生的眼中:在微生物角膜炎中的角膜微生物组
Susanna Sagerfors1, Sofie Edslev2, Birgitta Ejdervik Lindblad3
1Department of Ophthalmology, Faculty of Medicine and Health, Örebro University, SE 701 82, Örebro, Sweden. susanna.sagerfors@regionorebrolan.se.
概括
在培养负微生物角质炎病例中,有针对性的测序确定了病原体. 隐形眼镜的佩戴是一个主要的风险因素,影响这些严重的眼部感染的细菌组成和培养结果.
科学领域:
- 眼科医生 眼科 眼科
- 微生物学 微生物学
- 遗传学 是一个遗传学.
背景情况:
- 微生物角膜炎是一种严重的眼部感染,通常通过角膜培养来诊断.
- 培养方法可能是有限的,特别是在先前使用了抗生素或细菌负载较低的情况下.
- 有针对性的测序提供了一个互补的诊断方法.
研究的目的:
- 在微生物角质炎中使用向的16S rRNA基因测序来表征细菌病原体.
- 确定影响传统角膜培养成功的因素.
- 为了比较隐形眼镜佩戴者与非佩戴者的细菌群落.
主要方法:
- 对94名微生物角质炎患者的前性研究.
- 用标准培养和向16S rRNA基因测序 (V3-V4区域) 分析的角膜样本.
- 多变量回归分析以确定影响培养结果的因素.
主要成果:
- 隐形眼镜的佩戴 (73%) 是最常见的风险因素,与不同的细菌特征相关 (葡萄球菌,Corynebacterium,Stenotrophomonas).
- 测序检测到38%的培养负病例中的病原体.
- 年龄,细菌密度和先前的抗生素治疗影响了培养成功.
结论:
- 有针对性的测序增强了培养负微生物角质炎的病原体鉴定.
- 了解隐形眼镜佩戴等风险因素对于治疗细菌性角膜炎至关重要.
- 这种方法为角膜感染的微生物景观提供了宝贵的见解.
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