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相关概念视频

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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智能手机集成的纸质生物传感器用于敏感的度乙醇量化.

Mallesh Santhosh1, Tusan Park2,3

  • 1Smart Agriculture Innovation Center, Kyungpook National University, Daegu, Republic of Korea.

Mikrochimica acta
|November 22, 2023
PubMed
概括
此摘要是机器生成的。

本研究介绍了一种便携式,无仪器的方法,使用度纸质分析装置 (fPAD) 和智能手机进行精确的乙醇量化. 开发的fPAD具有高灵敏度和稳定性,非常适合在现场进行食品质量控制.

关键词:
乙醇生物传感器生物传感器基于纸张的测定方法.便携式光检测仪 便携式光检测仪基于智能手机的传感器葡萄酒的分析.

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科学领域:

  • 分析化学 分析化学
  • 生物技术是生物技术.
  • 材料科学 材料科学 材料科学

背景情况:

  • 准确的乙醇量化对于食品和饮料质量控制至关重要.
  • 现有的方法通常需要昂贵的仪器仪表和实验室设置.
  • 需要便携式,具有成本效益和用户友好的分析工具.

研究的目的:

  • 开发和验证一种新的基于纸张的度分析装置 (fPAD),用于无仪器的乙醇量化.
  • 将fPAD与智能手机技术集成,以提高便携性和可访问性.
  • 在现实世界样本中评估开发的fPAD的性能,稳定性和适用性.

主要方法:

  • 作为生物识别元素,在基托改性纸上固定NAD+依赖的酒精脱酶.
  • 使用酶生成的NADH作为光探针用于乙醇检测.
  • 使用UV-LED开发3D打印成像设置,用于快速光图像捕获.
  • 使用标准乙醇溶液验证fPAD并分析各种葡萄酒样本.

主要成果:

  • 开发的fPAD显示了对乙醇检测的高灵敏度,线性范围从17μM到8.75μM (R2=0.99).
  • 在红酒,白酒,酒和泡酒样本中成功量化了乙醇.
  • fPADs表现出极好的可重复性和酶稳定性,保持至少28天的活性.

结论:

  • 拟议的fPAD为现场乙醇量化提供了一个简单,负担得起和便携的解决方案.
  • 这项技术适用于食品质量控制应用,可实现快速准确的分析.
  • 与智能手机技术的整合提高了分析设备的可访问性和实用性.