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基因组甲基化和脂质新陈代谢参与GA诱导的玉米氨酸层PCD,正如转录组分析所揭示的那样

Yequn Wu¹, Jiaqi Hou¹, Ruifei Ren¹

¹State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

BMC plant biology

|November 22, 2023

概括

吉伯雷林 (GA) 诱导了植物氨酸层中的编程细胞死亡 (PCD). 这项研究揭示了表观遗传修饰,特别是DNA甲基化和基因素乙化,以及脂质代谢和ROS信号传递,是GA诱导的aleuronePCD的关键调节者.

科学领域:

植物生物学植物生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

植物种子中的阿鲁细胞在发芽过程中经历了编程细胞死亡 (PCD),这是由吉伯雷林 (GA) 触发的过程.
调控GA诱导的氨酸PCD的分子机制和信号通路尚未完全理解.
基因组脱乙酶 (HDACs) 已涉及GA诱导的阿勒类PCD.

研究的目的:

调查表观遗传修饰在调节阿勒PCD中的作用.
通过全球转录组分析,识别参与GA诱导的aleurone PCD的关键基因和途径.

主要方法:

全球比较转录组分析用GA或基因素乙酶 (HAT) 抑制剂治疗的无胚胎阿勒.
差异表达基因 (DEGs) 的分析及其在生物过程中的参与.
抑制DNA甲基转移酶和评估亚勒的PCD.

主要成果:

总共有7,919个DEG被确定,其中2,554个是GA和HAT抑制剂治疗中共同的.
DEGs与DNA甲基化,脂质代谢和活性氧物种 (ROS) 信号传递有关.
抑制DNA甲基化防止了aleurone PCD,而GA/HAT抑制诱导了 lipoxygenase 表达和脂质降解,独立于DNA甲基化. 抑制DNA甲基化影响了ROS相关的基因表达和过氧化 (H2O2) 生产.

结论:

在阿勒中,GA诱导的HDAC活性通过氧化酶基因调节导致脂质分解.
增加的DNA甲基化积极介导H2O2的生产.
DNA甲基化和脂质代谢途径在玉米中形成一个复杂的信号网络.

关键词:

通过DNA甲基化.在H2O2中,H2O2是H2O的.这就是HDACs.脂质代谢脂质代谢是一种玉米的艾鲁隆 (aleurones) 是一种编程细胞死亡 (PCD)

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