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菌 (Enterococcus faecium) 抑制NF-κB/NLRP3/IL-1β信号通路,并对抗沙门氏菌介导的炎症反应

Dechun Chen^1,2, Xiaodong Liang¹, Jiangying Lei¹

¹College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, China.

Future microbiology

|November 23, 2023

概括

菌菌 (Enterococcus faecium) 益生菌通过增强肠道屏障的完整性和减少炎症来防止沙门氏杆菌 (Salmonella typhimurium) 感染. 这种益生菌调节关键的炎症通路,减轻小鼠的组织损伤.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
胃肠病学胃肠病学

背景情况:

沙门氏菌 Typhimurium 感染会导致严重的胃肠道损伤.
维持肠道屏障功能对于防止病原体入侵和炎症至关重要.
益生菌为减轻传染病影响提供了一个潜在的战略.

研究的目的:

在小鼠模型中研究Enterococcus faecium对沙门氏菌typhimurium感染的保护作用.
阐明E. faecium的保护作用背后的分子机制,重点关注炎症反应和肠道屏障完整性.

主要方法:

肠道组织的组织学检查,以评估损伤.
实时定量PCR和免疫组织化学分析基因和蛋白质表达.
对炎症性细胞因子 (IL-6,TNF-α,IFN-γ) 和信号通路组件 (NF-κB/NLRP3/IL-1β) 的评估.

主要成果:

E. faecium增强了Claudin-1的表达,加强了紧密的结节和肠道屏障功能.
这种益生菌抑制了NF-κB/NLRP3/IL-1β信号通路.
E. faecium显著降低了促炎性细胞因子 (IL-6,TNF-α,IFN-γ) 和相关分子 (TLR4,MYD88) 的释放,减轻了十二指肠,阴和结肠中的组织损伤.

结论:

菌 (Enterococcus faecium) 在小鼠中表现出显著的保护作用,防止沙门氏菌 (Salmonella typhimurium) 引起的炎症损伤.
益生菌的机制包括加强肠道屏障和调节NF-κB/NLRP3/IL-1β信号通路.
通过减轻肠道炎症,E. faecium 作为治疗沙门氏菌感染的潜在治疗剂.

关键词:

肠球菌 (Enterococcus faecium) 是一个有毒的菌株.在 NF-κB/NLRP3/IL-1β 中.沙门氏菌是一种沙门氏菌.没有了,没有了,没有了.

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