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马特林通过调节circ_0055976/miR-1179/乳酸脱酶A轴来抑制肝细胞癌瘤发生

Xinfeng Chang¹, Hongwei Li², Zhengchun Huang²

¹Department of human anatomy, Jiangsu Vocational College of Medicine, Yancheng, China.

Environmental toxicology

|November 23, 2023

概括

马特林通过向circ_0055976/miR-1179/LDHA通路来抑制肝细胞癌 (HCC). 这项研究揭示了Matrine.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

马特林在肝细胞癌 (HCC) 中表现出抗癌特性.
循环RNA (circ_0055976) 在HCC中被上调.
在HCC瘤发生过程中,Matrine和circ_0055976之间的相互作用需要进行研究.

研究的目的:

调查HCC中Matrine和circ_0055976的功能作用和分子机制.
为了阐明Matrine,circ_0055976和HCC进展之间的关系.

主要方法:

在体外测试:细胞计数套件-8,EDU,殖民地形成,转孔测试扩散和入侵.
评估有氧糖解,基因/蛋白质表达通过qRT-PCR和西式涂抹.
双露西法酶记者测定和老鼠异种移植模型,以确认分子相互作用和体内疗效.

主要成果:

在实验室中,Matrine抑制了HCC细胞的增殖,入侵和无氧糖解.
Circ_0055976,在HCC中高度表达,被Matrine降低;它的过度表达逆转了Matrine的影响.
确定了circ_0055976/miR-1179/LDHA轴,Matrine的作用依赖于这个途径;Matrine在体内抑制瘤生长,通过circ_0055976沉默增强.

结论:

马特林通过调节circ_0055976/miR-1179/LDHA轴来对HCC产生抗癌作用.
这一途径为Matrine在HCC治疗中提供了一个新的治疗点.

关键词:

在HCC中,HCC是HCC.马特里尼 (Matrine) 是一个母乳.有氧糖解有氧糖解.环_005597676 时间时间时间这是入侵的入侵.它们的扩散和扩散.

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