在老鼠和小鼠中,对每日编程的抵抗运动的保存和特定物种的转录反应
Mark R Viggars1,2,3, Hazel Sutherland1, Christopher P Cardozo4,5
1Research Institute for Sport & Exercise Science, Liverpool John Moores University, Liverpool, UK.
概括
小鼠和老鼠对运动的基因反应类似,但小鼠优先考虑能量补充而不是肌肉生长. 这就解释了为什么小鼠不会像老鼠那样表现出过度缩.
科学领域:
- 运动生理学 运动生理学
- 分子生物学分子生物学
- 进行比较的基因组学.
背景情况:
- 小鼠是研究骨肌适应运动的基因调节的常见模型.
- 用电神经刺激进行阻力训练会在老鼠中诱导肌肉缩,但不会在小鼠中.
研究的目的:
- 为了将急性转录反应与小鼠和老鼠相同的神经刺激范式进行比较.
- 研究物种之间的肌肉生长反应差异的分子基础.
主要方法:
- 在自由生活的小鼠和老鼠中,利用植入式脉冲发生器进行每日抗力训练与电神经刺激.
- 在训练2,10和20天后,在运动1小时后评估前肌的急性转录变化.
- 采用RNA测序来分析基因表达模式并识别差异调节的途径.
主要成果:
- 在两种物种中,在时间调节应激反应,原平衡,核糖体子单元,自和焦点粘附途径方面表现出强烈的一致性.
- 在小鼠和老鼠之间观察到能量代谢途径的相反调节,包括碳代谢,氧化酸化和氨基酸降解.
- 在大鼠中发现了能量代谢途径的抑制,与小鼠的上调调节形成鲜明对比.
结论:
- 虽然骨肌肉生长途径在小鼠和老鼠之间显示出很高的相似性,但小鼠优先考虑能量代谢和燃料储存补充.
- 在小鼠中,这种对能量代谢的优先考虑可能解释了缺乏显著的肌肉生长反应,尽管与生长相关的途径具有类似的转录激活.
- 这些发现突出了运动期间代谢适应策略的特定物种差异,影响骨肌肉缩.
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