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在无氧氨基生物中对线粒体相关器官的比较分析

Kristína Záhonová^1,2,3,4, Zoltán Füssy¹, Courtney W Stairs^5,6

¹Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czechia.

Microbial genomics

|November 23, 2023

概括

这项研究揭示了阿尔卡莫贝的共同祖先具有有限的线粒体功能,但拥有硫酸盐激活和铁硫 (Fe-S) 组装的关键途径. 这些有机体的新陈代谢能力在Archamoebae类中显著不同.

科学领域:

细胞微生物学的微生物学
机体细胞的进化过程
生物化学生物化学

背景情况:

阿卡莫贝群是居住在无氧环境中的原生体,具有适应无氧功能的线粒体相关器官 (MROs).
了解MROs的进化轨迹和代谢能力对于理解早期真核生物进化至关重要.

研究的目的:

重建和比较八种阿卡莫贝的MRO蛋白质组,以推断祖先的MRO功能.
为了研究MROs在Archamoebae类的关键代谢途径的进化动态.

主要方法:

从基因组数据中在体中重建MRO蛋白质组.
对不同Archamoebae物种的蛋白质含量和代谢途径进行比较分析.

主要成果:

祖先Archamoebae MRO具有减少的蛋白质转位机械,电子运输链和TCA循环,但具有硫酸盐激活和MIS类型的Fe-S组件.
类内存在显著的代谢变异,保留了甘氨酸裂变系统 (除Entamoeba外) 和可变的pyruvate代谢和硫酸盐激活.
在Mastigamoebidae和Pelomyxidae中,Fe-S组装系统经历了重复,潜在的双重定位在Entamoebidae和Rhizomastixidae中促进了组装.

结论:

阿卡梅巴MROs从一个简化的祖先进化,具有专门的无氧功能.
在MRO中,代谢可塑性是显著的,但环境因素似乎不是MRO减少的强有力的驱动因素.

关键词:

无氧生态系统 (anaerobiosis) 是一种无氧生态系统.进行比较的基因组学.与线粒体相关的器官减少进化的进化.

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