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综合性lncRNA,circRNA和mRNA分析揭示了六种法医体液/组织的表达特征

Zhiyong Liu^1,2, Jingyi Yang^1,2, Nana Wang^1,2

¹Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.

International journal of legal medicine

|November 23, 2023

概括

这项研究全面分析了法医样本中的RNA,识别了新的组织特异标记物和转录的微型类型,用于体液识别和个体供体追踪.

科学领域:

法医科学法医科学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

RNA的组织特异表达为法医体液/组织识别 (BFID) 提供了潜在的潜力.
较长的RNA (例如mRNA) 可能含有更多的多态位点用于个人识别.
在法医背景下对RNA资料的全面表征是有限的.

研究的目的:

在各种人体液体和组织中全面分析mRNA,lncRNA和circRNA配置文件.
为法医应用识别新型体液/组织特异性RNA标记物.
为了探索个体识别的转录组微型 (tMHs).

主要方法:

30个法医样本的转录组测序 (血液,精液,唾液,阴道分泌物,皮肤).
对mRNA,lncRNA和circRNA的表达和分布进行生物信息分析.
从RNA转录中对SNP和InDels进行基因定型,以识别tMHs.

主要成果:

检测到了90,305个mRNA,102,906个lncRNA (19,549个新型RNA) 和40,204个circRNA.
确定了许多新的体液/组织特异性RNA标记物.
发现了21,611个多SNP和4,471个多InDel tMHs.

结论:

提供了对法医样本中转录组概况的详细了解.
新型RNA标记物和tMH为体液来源追踪和个人识别提供了新的可能性.
这项研究推进了基于RNA的法医分析.

关键词:

法医污点在法医污点上.大规模并行测序 (MPS) 是一种转录的微哈普罗型 (tMH)环RNA 环RNA 是一个环RNA.在ncRNA中,我们可以它们是mRNARNA.

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