与WUSCHEL相关的家庭盒转录因子SlWOX13调节番茄果实成熟的过程
Guoxiang Jiang1,2,3, Zhiwei Li1,2,3, Xiaochun Ding1,2
1State Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Plant physiology
|November 23, 2023
概括
一种转录因子SlWOX13积极调节番茄果实成熟的过程. 它控制乙烯合成,胡卜素积累和关键的成熟相关的转录因子,影响整体的成熟过程.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 发展水果发展水果发展
背景情况:
- 果实成熟是一个复杂的,基因控制的过程.
- 转录因子 (TF) 是植物发育的关键调节者.
- 与WUSCHEL相关的家庭盒 (WOX) TFs在水果成熟中的作用在很大程度上是未知的.
研究的目的:
- 为了研究SlWOX13在番茄果实成熟中的功能.
- 阐明SlWOX13调节成熟的分子机制.
主要方法:
- 在番茄 (Solanum lycopersicum) 中,基因过度表达和功能丧失突变生成.
- 用于基因鉴定的RNA测序 (RNA-seq) 和染色体免疫沉测序 (ChIP-seq).
- 电泳运动转移试验 (EMSA),RT-qPCR,双露西法酶记者试验和ChIP-qPCR用于验证.
主要成果:
- 过度表达SlWOX13加速了成熟,而突变延迟了成熟.
- SlWOX13调节乙烯的合成和胡卜素的积累.
- SlWOX13直接针对涉及乙烯和胡卜素通路的基因,并与关键的成熟TFs (RIN,NOR,NAC4) 相互作用.
结论:
- SlWOX13是番茄果实成熟的积极调节者.
- 它通过调节乙烯合成和信号通路来起作用.
- SlWOX13还通过对其他关键成熟相关TFs的转录调节来影响成熟.
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