RNA m6A 修饰在玉米核发育过程中促进了 DNA 甲基化
Jin-Hong Luo1, Ting Guo2, Min Wang2
1Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Plant physiology
|November 23, 2023
概括
玉米 (Zea mays) 的研究揭示了N6-甲基氨酸 (m6A) RNA和5-甲基氨酸 (5mC) DNA修饰之间的交叉声. 这种相互作用影响基因表达和玉米核发育,为表观遗传调节提供了新的见解.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因规则 基因规则
背景情况:
- 在mRNA中的N6-甲基氨酸 (m6A) 和DNA中的5-甲基氨酸 (5mC) 是调节基因表达和植物发育的关键表观遗传标记.
- 在植物中m6A和5mC之间的机械相互作用在很大程度上仍然未被描述.
研究的目的:
- 研究玉米 (Zea mays) 中的m6ARNA修饰和5mCDNA甲基化之间的交叉关系.
- 阐明植物发育中的m6A和5mC通路之间的相互作用的分子机制.
主要方法:
- 研究了玉米中mRNA腺甲基酶 (ZmMTA) 和DNA甲基化1 (ZmDDM1) 的降低之间的相互作用.
- 分析了玉米基因中m6A修饰水平和DNA甲基化状态之间的相关性.
- 检查了ZmMTA和ZmDDM1功能障碍对玉米胚胎发生,内精发育和DNA甲基化模式的发展后果.
主要成果:
- 在玉米中发现了m6A和5mC之间的交叉,这种交叉是由ZmMTA和ZmDDM1.1.的相互作用介导的.
- 与未经修改的基因相比,具有m6A修改的基因表现出显著更高的DNA甲基化水平.
- ZmMTA功能障碍导致m6A修饰基因的发育停止和CHH甲基化减少,而ZmDDM1功能障碍对ZmMTA活性的影响最小.
结论:
- 在玉米核发育中建立了m6ARNA修饰和5mCDNA甲基化之间的直接联系.
- 在植物中通过RNA修饰和DNA甲基化对基因表达的协调调节提供了新的见解.
相关概念视频
Epigenetic Regulation
3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.0K
Histone Modification
13.3K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.3K
Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
Chromatin Modification in iPS Cells
1.7K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.7K
Spreading of Chromatin Modifications
8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.3K
RNA Stability
33.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.6K


