哺乳动物卵细胞在生殖囊泡和转化II阶段的差异化转录使用
Thanida Sananmuang1, Denis Puthier2, Catherine Nguyen2
1Rajamangala University of Technology Tawan-OK, Faculty of Veterinary Medicine, Chonburi, Thailand.
Theriogenology
|November 23, 2023
概括
这项研究揭示了在哺乳动物中卵细胞成熟期间转录异型变化的关键基因. 这些发现为改善辅助生殖技术提供了潜在的新标记.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 辅助生殖技术 (ART) 在mRNA测序方面取得了进展,但专注于基因表达,而不是转录异型.
- 了解转录异形表达对于卵细胞细胞机制至关重要.
- 差异性转录使用 (DTU) 分析可以弥合这一知识差距.
研究的目的:
- 对6种哺乳动物物种的卵细胞mRNA-Seq数据集进行全面的DTU分析.
- 为了确定参与卵细胞成熟的保存的跨物种DTU基因.
- 通过基因本体学 (GO) 丰富分析来探索这些已识别的基因的功能作用.
主要方法:
- 利用了来自牛,驴,马,人类,老鼠和猪的生殖囊泡 (GV) 和元相II (MII) 卵细胞的mRNA-Seq数据集.
- 进行了DTU分析,以识别具有转录异型变异的基因.
- 在跨物种DTU基因上进行了GO术语丰富分析.
主要成果:
- 确定了十个保存的跨物种DTU基因 (ABCF1, CDC6, CFAP36, CNOT10, DNM3, IWS1, NBN, NDEL1, RAD50, ZCCHC17) 在至少五种物种中存在 (FDR≤0.05).
- GO分析将这些基因与RNA和细胞循环控制机制联系起来.
- 在各种哺乳动物中,DTU基因在卵细胞成熟中发挥着保留的作用.
结论:
- 保存的DTU基因在哺乳动物卵细胞成熟中起着至关重要的作用.
- 转录异形分析提供了比整体基因表达更深入的见解.
- 这些发现可能会导致新的转录异形标志物用于增强ART.
相关概念视频
Meiosis II
183.5K
Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...
183.5K
Oogenesis
63.8K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.8K
What is Meiosis?
224.0K
Meiosis is the process by which diploid cells divide to produce haploid daughter cells. In humans, each diploid cell contains 46 chromosomes, half from the mother and half from the father. Following meiosis, the resulting haploid eggs or sperm only contain 23 chromosomes; however, each of these chromosomes contains a unique combination of parental information that results from the meiotic process of crossing over.
224.0K
Meiosis vs. Mitosis
56.6K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
56.6K
Meiosis I
193.7K
Meiosis is a carefully orchestrated set of cell divisions, the goal of which—in humans—is to produce haploid sperm or eggs, each containing half the number of chromosomes present in somatic cells elsewhere in the body. Meiosis I is the first such division, and involves several key steps, among them: condensation of replicated chromosomes in diploid cells; the pairing of homologous chromosomes and their exchange of information; and finally, the separation of homologous chromosomes by...
193.7K
Cellular Differentiation
2.7K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
A zygote is a...
2.7K


