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一种RNA螺旋酶CaRH57有助于胡对热应激的耐受性

Min Zheng¹, Yu Song¹, Lingyu Wang¹

¹School of Life Sciences, Southwest University, Chongqing, China.

Plant physiology and biochemistry : PPB

|November 23, 2023

概括

胡RNA螺旋酶CaRH57通过帮助mRNA前拼接来增强耐热性. 这种DEAD-box蛋白对于植物在热应激条件下生存至关重要,防止死亡和ROS积累.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
压力生理学压力生理学

背景情况:

RNA螺旋酶 (RHs) 对于RNA代谢和植物的压力耐受性至关重要.
热应激 (HS) 严重影响植物细胞,抑制生长和发育.
胡 (Capsicum annuum) 是一个全球重要的作物,容易受到热应激.

研究的目的:

为了研究死盒RNA酶CaRH57在热应激下的胡植物中的作用.
确定CaRH57是否可以功能性地取代其Arabidopsis thaliana同类物 (AtRH57).
阐明CaRH57赋予耐热性的分子机制.

主要方法:

来自胡的CaRH57的识别和特征.
在Atrh57-1突变体中过度表达CaRH57,以评估功能补充.
在体外测试以确认RNA酶活性.
使用烟草虫病毒 (TRV2) 评估其在耐热性中的作用.
分析植物表型,活性氧物种 (ROS) 积累和在热应激下进行的mRNA前拼接.

主要成果:

热应激诱导了CaRH57的表达.
过度表达CaRH57挽救了Atrh57-1的葡萄糖敏感表型,表明功能保存.
在实验室中,CaRH57表现出RNA螺旋酶活性,并局部化到细胞核中.
降低CaRH57导致热耐受性受损,亡增加和ROS积累增加.
淘汰植物显示CaHSFA1d和CaHSFA9d前mRNA的异常拼接,并在热应激下降成熟mRNA水平.

结论:

CaRH57是一种关键的RNA酶,它赋予胡的耐热性.
在热应激反应基因的适当的mRNA前拼接中,CaRH57起着至关重要的作用.
CaRH57是提高作物耐热应激能力的潜在目标.

关键词:

无生物应激应激是一种非生物应激.热应激是一种热应激.胡胡胡是一个它们是RNA螺旋酶.这就是ROSOS ROS.

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