在中,前垂体腺分泌的益生菌的分子形式鉴定
Norio Kansaku1, Takeshi Ohkubo2
1Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, Fuchinobe, Chuo-ku, Sagamihara-Shi, Kanagawa 252-5201, Japan.
General and comparative endocrinology
|November 23, 2023
概括
这项研究表明,化期间分泌的益乳素 (PRL) 呈现出多种分子形式,包括糖化和非糖化分离形式. 这些变异与生殖阶段和养行为有关.
科学领域:
- 生殖内分泌学 生殖内分泌学
- 鸟类生物学 鸟类生物学
- 分子内分泌学分子内分泌学
背景情况:
- 内分泌变化对于鸟类的繁殖至关重要,前乳素 (PRL) 与化和养行为密切相关.
- 虽然 pituitary PRL 的形式是已知的,但在生殖周期期间分泌的 PRL 分子形式仍然没有特征.
研究的目的:
- 为了确定在丝母的繁殖周期期间分泌的益乳素 (PRL) 的分子形式.
- 为了将行为变化与生殖和PRL分泌的生理阶段相关联.
主要方法:
- 在1-2年内对10只丝母进行行为监测,使用视频记录来追踪生理状态和化行为.
- 前垂体腺培养在四个生殖阶段 (早产非产卵,产卵,转化,化) 用血管活性肠 (VIP).
- 二维蛋白质电泳以分析分泌的PRL分子形式.
主要成果:
- 巢穴占用率的可重复变化,表明即将发生的化,发生在发病前7-10天.
- 分泌的PRL表现出多个糖化PRL (G-PRL) 异型,其异电点 (pI) 不同.
- 非糖化PRL (NG-PRL) 显示了一个主要信号,但在垂体中检测到3-4种异型,这表明分泌的PRL在翻译后发生了修改.
结论:
- 分泌的PRL,特别是在转化和化阶段,包括各种异构体.
- 这些异构体是翻译后糖化和化的结果,有助于增加鸟类繁殖中PRL信号的复杂性.
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