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将Neuropixels探头插入醒着的子皮层:两个探头,两个方法.

Tomoyuki Namima1, Erin Kempkes2, Bob Smith3

  • 1Department of Biological Structure and Washington National Primate Research Center, University of Washington, Seattle, WA 98195, USA; Graduate School of Frontier Biosciences, Osaka University, and Center for Information and Neural Networks, National Institute of Information and Communications Technology, Suita, Osaka 565-0871, Japan.

Journal of neuroscience methods
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概括
此摘要是机器生成的。

研究人员开发了新的方法,将Neuropixels探针插入灵长类动物的大脑,克服了较厚的硬度所带来的挑战. 这些技术可以在清醒的子中进行可靠的长期神经生理记录.

关键词:
慢性记录室的记录室高密度探测器的探测器多接触线性探针多接触线性探针非人类灵长类神经生理学

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科学领域:

  • 神经科学是一个神经科学.
  • 灵长类动物生理学
  • 神经技术的神经技术

背景情况:

  • 神经像素探针对于动物神经生理学至关重要.
  • 灵长类 dura 对探头插入具有重大障碍.
  • 现有的方法不足以进行灵长类大脑记录.

研究的目的:

  • 开发和验证急性Neuropixels探头插入醒着的子子的方法.
  • 为了克服与灵长类长质厚度相关的挑战.
  • 为了实现非人类灵长类动物的高产量,长期的神经生理记录.

主要方法:

  • 开发了用于微型神经像素1.0 (IMEC) 探针的持续眼球方法.
  • 为更厚的Neuropixels NP1010探头创建了一个人造的dura系统.
  • 实施的手术准备和探针插入/移除技术,用于清醒的.

主要成果:

  • 成功地将Neuropixels探针插入7个记录室中的3只动物中.
  • 在几个月内实现了一致的,高产量的录音.
  • 证明了dural-eyelet和人工dura方法的有效性.

结论:

  • 开发的方法有助于对灵长类动物进行可靠的Neuropixels探头插入.
  • 这些技术对于推进灵长类神经生理学研究非常有价值.
  • 这些方法支持灵长类研究的硬件,手术准备和探针移除策略.