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基于的衍生物化,用于在质谱成像中快速标记和增强检测硫醇

Shuai Huang¹, Haiyang Wang², Xinxin Liu³

¹CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR China; University of Chinese Academy of Science, Beijing, 100039, PR China.

Analytica chimica acta

|November 23, 2023

概括

这项研究引入了一种新的质谱成像方法,用于绘制大脑组织中醇化合物的图像. 该技术能够对这些关键分子进行敏感和定量检测,有助于抑郁症研究.

科学领域:

生物化学生物化学
神经科学是一个神经科学.
分析化学分析化学

背景情况:

内源性抗氧化剂,如谷氨 (GSH) 具有关键的醇功能组.
通过MALDI分析微量硫醇是很困难的,因为不稳定性和低电离.
绘制脑组织中醇分布的地图对于理解神经系统过程至关重要.

研究的目的:

开发一种质谱成像 (MSI) 方法,用于绘制大脑组织中醇化合物的图像.
为了能够选择性和快速检测GSH合成和代谢途径相关的代谢物.
在慢性社会失败压力 (CSDS) 抑郁模型中证明该方法的能力.

主要方法:

使用衍生试剂MTMP和MTPP用于醇组的共价电荷标记.
采用了矩阵辅助激光脱离离子化质谱成像 (MALDI-MSI).
验证了N-乙半氨酸的定量检测,检测极限为0.05 ng.

主要成果:

成功地在脑组织部分上绘制了醇化合物的地图.
已证明可以选择性和快速检测硫醇代谢产物.
在复杂的生物样本中实现了对定量醇分析的高灵敏度.

结论:

开发的醇特异性MSI方法为分析大脑组织中的醇化合物提供了一种敏感和定量方法.
这种技术有助于在神经疾病模型中研究GSH代谢和相关途径.
该方法有望促进我们对抑郁症和其他与醇相关的神经系统疾病的理解.

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