动态控制使得脱离混合的生物分子凝聚物的形成成为可能
Andrew Z Lin1, Kiersten M Ruff2, Furqan Dar2
1Division of Biology and Biomedical Sciences, Plant and Microbial Biosciences, Washington University in St. Louis, St. Louis, MO, 63130, USA.
Nature communications
|November 23, 2023
概括
生物分子冷凝剂可以通过动态控制来实现不同的组成. RNA分子动力学,特别是它们的捕获,驱动凝结物形成,在体内影响细胞组织.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 分子生物学分子生物学
背景情况:
- 生物分子凝聚物对于细胞组织至关重要.
- 构成上不同的冷凝液的形成还没有完全理解.
- 像Whi3这样的RNA结合蛋白在凝结物形成中起作用.
研究的目的:
- 为了研究规范组成上不同的生物分子凝聚物的形成的物理原理.
- 探索热力学和动力学因素在凝结物形成中的作用.
- 了解动态控制如何影响体外和体外的冷凝化合物组成.
主要方法:
- 利用基于热力学考虑的物理框架.
- 在Whi3蛋白和RNA分子的二元和三元混合物中进行了体外实验.
- 在同时和延迟添加组件的条件下分析了凝结物形成.
- 通过同步RNA生产来研究体内凝聚物形成.
主要成果:
- 试验室凝聚物主要通过二进制Whi3-RNA混合物的异型相互作用形成.
- 凝结体内的RNA分子可以变得动态被捕.
- 延迟添加RNA组件会导致组成上不同的冷凝物,而同时添加会产生混合良好的冷凝物.
- 在体内同步RNA生产有利于混合良好的凝聚物,而不是不同的凝聚物.
结论:
- 通过动态控制可以实现凝结物组成,特别是受RNA动态停止的影响.
- 添加组件的时间显著影响了冷凝液异质性.
- 活体RNA生产同步影响凝聚物混合,这表明动态控制是细胞组织的关键因素.
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