分泌的PAMP诱导StPIP1_1激活了土豆中的免疫反应
Linda Nietzschmann1, Ulrike Smolka1, Elvio Henrique Benatto Perino1
1Department Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle (Saale), Germany.
Scientific reports
|November 23, 2023
概括
用Pep-13治疗的土豆植物激活了1200多个基因,包括StPIP1_1.1. 这种增强了植物对病原体的防御反应,如Phytophthora infestans.
科学领域:
- 植物免疫力 植物免疫力
- 分子植物病理学 分子植物病理学
- 植物的信号传输.
背景情况:
- 与病原体相关的分子模式 (PAMPs),如Pep-13,触发了植物的防御反应.
- 植物虫 (Phytophthora infestans) 导致晚期病,这是一个毁灭性的土豆疾病.
- 由PAMP诱导的分泌 (PIPs) 参与植物防御信号传递.
研究的目的:
- 为了研究马PAMP诱导的分泌,StPIP1_1,在植物防御中的作用.
- 为了确定StPIP1_1.1.的感知机制和信号通路.
- 了解StPIP1_1是如何放大防御反应的.
主要方法:
- 用Pep-13和Phytophthora infestans治疗的土豆植物中的基因表达分析.
- 使用mCherry聚变蛋白确定StPIP1_1定位的共聚焦显微镜.
- 合成治疗,以评估StPIP1_1对基因表达和氧化突发的影响.
- 在Arabidopsis中进行功能分析,以研究StPIP1_1与受体激酶的相互作用.
主要成果:
- 在土豆植物中,Pep-13治疗诱导了超过1200个基因,包括StPIP1_1.
- StPIP1_1被分泌到细胞质中,其表达是由P. infestans感染引起的.
- 对外应用StPIP1_1衍生的引发了防御基因表达和氧化突发,依赖于StSERK3A/B.
- StPIP1_1以RLK7-依赖的方式诱导了Arabidopsis中的FRK1表达,并且还诱导了土豆中的同源RLK.
结论:
- StPIP1_1作为一种内源性,可以放大植物的防御反应.
- 对StPIP1_1的感知涉及一个包括StSERK3A/B.在内的受体综合体.
- 这些发现揭示了植物免疫中一种新的质介导放大循环.
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