相关实验视频

Updated: Jul 10, 2025

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Published on: February 3, 2023

长期的HIF-1α稳定会减少呼吸,促进细胞和导致视网膜细胞死亡

Nana Yaa Nsiah^1,2, Autumn B Morgan¹, Nina Donkor¹

¹Department of Pharmaceutical Sciences, North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, USA.

Scientific reports

|November 23, 2023

概括

低氧诱导因子1-α (HIF-1α) 的慢性激活会引发代谢变化和视网膜细胞死亡,这表明它可以独立于高眼压驱动青光眼病理.

科学领域:

眼科医生眼科眼科
代谢途径代谢途径
细胞生物学细胞生物学

背景情况:

玻璃眼和眼睛高血压可能导致缺氧,导致HIF转录因子的激活和代谢转移到糖解.
这种新陈代谢重编程可能会导致与玻璃眼相关的病理.

研究的目的:

调查慢性HIF激活是否可以独立于眼睛高血压,启动与青光眼相关的病理.
分析持续在视网膜中稳定HIF-1α的代谢和细胞后果.

主要方法:

在小鼠中诱导HIF-1α稳定,使用prolyl氧酶抑制剂Roxadustat2周和4周.
用免疫光,毛细血管电泳和酶活性测试来评估视网膜变化.
细胞亡和视网膜质细胞功能通过TUNEL测定和PERG振幅进行了评估.

主要成果:

罗沙杜沙特治疗稳定了HIF-1α,上调了糖解相关蛋白质 (GLUT3,PDK-1) 和酶活性.
线粒体标志物 (SDH,MTCO1,酸盐合成酶) 降低,而线粒体菌增加.
观察到显著的视网膜细胞亡,PERG振幅下降,AMPK激活增加和质缩.

结论:

仅仅慢性HIF-1α稳定对视网膜代谢平衡和细胞存活是有害的.
这些发现表明,HIF-1α激活可以独立于眼睛高血压驱动青光眼病理.
由HIF-1α诱导的代谢重编程有助于视网膜细胞死亡和功能障碍.

更多相关视频

Author Spotlight: Extended Oxygen Consumption Measurement in Retinal Pigment Epithelium Using Resipher

Author Spotlight: Extended Oxygen Consumption Measurement in Retinal Pigment Epithelium Using Resipher

Published on: August 16, 2024

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...