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通过使用农业副产品作为基质的Corynebacterium glutamicum (ATCC 13032) 固态发酵生产L-lysine

Egoamaka O Egbune^1,2, Theresa Ezedom³, Otuke B Odeghe³

¹Department of Biochemistry, Faculty of Science, Delta state University, P.M.B. 1, Abraka, Nigeria. egbuneegoamaka@gmail.com.

World journal of microbiology & biotechnology

|November 23, 2023

概括

这项研究表明,Corynebacterium glutamicum可以从农业废物中产生L-lysine. 用大象草提取物进行固态发酵可以提高产量,提供这种必需氨基酸的可持续本地来源.

科学领域:

生物技术是生物技术.
微生物学微生物学
生物化学工程生物化学工程

背景情况:

全球对L-氨酸的需求不断增长,需要可持续的本地生产方法.
对L-lysine的进口依赖可能在经济和后勤方面具有挑战性.
农业副产品代表了生物处理中未充分利用的资源.

研究的目的:

评估Corynebacterium glutamicumATCC 13032对从各种农业副产品中生产L-lysine的疗效.
为了优化L-lysine产量,使用固态发酵 (SSF) 与大象草提取物补充.
评估SSF对可溶性蛋白质含量和酶活性的影响.

主要方法:

棕核蛋糕,大豆蛋糕,花生蛋糕和米的固体发酵使用C. glutamicum (10^6 CFU/ml).
在室温下进行72小时的发酵,使用不同度的大象草提取物.
量化L-氨酸,可溶性蛋白质和酶活性 (氧氨酶,氨酶,蛋白酶).

主要成果:

农业副产品含有剩余的L-lysine,可以通过SSF增强.
用大象草提取物补充的花生蛋糕产生了最高的L-lysine度 (3.27 ± 0.02 mg/gds).
观察到可溶性蛋白质 (大豆蛋糕中的7.941±0.05毫克/日) 和酶活性显著增加 (p<0.05).

结论:

科里尼细菌谷氨基通过SSF有效地从农业副产品中生物生产L-lysine.
大象草提取物显著提高了L-lysine的产量,并提高了营养价值.
这项研究提出了一种可持续且具有成本效益的生物技术途径,用于本地L-lysine制造.

关键词:

农业副产品农业副产品在Corynebacterium glutamicum的研究中,我们发现对于L-lysine来说,这是一个非常重要的药物.固态发酵固态发酵是什么

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