在传染性牛角膜结膜炎的背景下,牛肉细菌眼表面微生物组的变化
Hannah B Gafen1, Chin-Chi Liu1, Nikole E Ineck1
1Department of Veterinary Clinical Sciences, Louisiana State University, Skip Bertman Drive, Baton Rouge, LA, 70803, USA.
Animal microbiome
|November 24, 2023
概括
传染性牛角膜结膜炎 (IBK) 涉及牛眼表面微生物组 (OSM) 的显著变化,其中增加了Moraxella bovoculi和Rothia nasimurium. 这些发现为新的IBK治疗策略提供了潜力.
科学领域:
- 兽医微生物学 兽医微生物学
- 细菌基因组学 细菌基因组学
- 牛的健康 牛的健康
背景情况:
- 传染性牛结膜炎 (IBK) 在牛群中造成重大经济损失.
- 了解细菌的眼表面微生物群 (OSM) 对于管理IBK至关重要.
- 以前的研究主要集中在Moraxella bovis作为致病剂.
研究的目的:
- 在正常眼睛和具有活性IBK的眼睛中全面描述牛菌OSM.
- 研究除了M. bovis之外的各种细菌在IBK.病变发生过程中的作用.
- 探索宿主因素和环境对OSM的影响.
主要方法:
- 从正常 (n=376) 和受IBK影响的 (n=228) 牛眼睛中采集的结膜样.
- 细菌培养以识别特定物种.
- 16S rRNA基因测序用于相对丰度 (RA) 分析.
- 实时聚合酶连锁反应 (RT-PCR) 用于半定量建模.
主要成果:
- 与正常眼相比,IBK眼睛中Moraxella bovoculi的发病率明显高于正常眼睛.
- 在正常眼睛中,包括Corynebacterium spp.在内的Actinobacteriota的相对丰度更高,而在IBK眼睛中,Rothia nasimurium的相对丰度更高.
- 通过RT-PCR建模,有效地区分了正常和IBK眼球微生物组,其中包括M. bovoculi,M. bovis和Staphylococcus spp. 这些都是关键指标.
- 阿尔法多样性没有显著差异,但β多样性因地理位置,年龄,性别和品种而有显著差异.
结论:
- 在IBK期间,牛的细菌OSM发生了显著的变化,不仅涉及M. bovis更多的细菌,特别是M. bovoculi和R. nasimurium.
- 在RA中Actinobacteriota的变化为新的IBK疗法提供了潜在的点.
- 通过RT-PCR建模可靠地区分IBK和正常的眼睛微生物组.
- 地理位置和牛的品种显著影响牛的细菌OSM.
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