通过对称的小的自我组装合成原蛋白基
I Caglar Tanrikulu1, Lianna Dang2, Lekha Nelavelli2
1Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|November 24, 2023
概括
研究人员使用粘性结尾对称自组装 (SESSA) 开发了合成原蛋白纳米纤维. 这种方法为组织工程创造了强大的,可重复的水凝,克服了动物衍生材料的局限性.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 聚合物化学 聚合物化学
背景情况:
- 像原蛋白这样的动物衍生水凝对于组织工程至关重要,但患有可再生性,免疫性和污染问题.
- 合成水凝通过化学定义提供解决方案,避免生物污染物和免疫反应.
- 设计合成原蛋白水凝是具有挑战性的,因为原蛋白的复杂的三螺旋结构.
研究的目的:
- 为了克服创造合成原蛋白水凝的设计挑战.
- 研究原仿真 (CMP) 自组合成纳米纤维和水凝的过程.
- 建立合成原蛋白水凝的合理设计方法.
主要方法:
- 建模对称关联状态用于短CMP的粘结式对称自组装 (SESSA).
- 利用SESSA来最大限度地提高纳米纤维形成的三重螺旋链相互作用.
- 在自组装的CMP中调查纤维间协会.
主要成果:
- 确定了33个残留的CMP,通过粘性末端自组装并形成水凝.
- 在多个规模的自我组装行为中表现出了显著的一致性.
- 在CMP的三螺旋架构和水凝特性之间建立了明确的联系.
结论:
- 塞萨是设计合成原蛋白水凝的有效和强大的方法.
- 这种方法可以合理设计具有可预测性质的生物材料.
- 合成原蛋白水凝可以克服组织工程中动物衍生的对应物的局限性.
相关概念视频
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Ribosomes
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Nucleic Acid Structure
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
DNA Structure
DNA has a double-helix structure. The...


