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以同位素编码的组织衍生用于定量质谱仪对生物组织中短链脂肪酸的成像

Yuhao Han¹, Xinyan Qu^1,2, Haoyuan Geng¹

¹Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.

Analytical chemistry

|November 24, 2023

概括

这项研究引入了一种新的成像方法,可视化肠道中的短链脂肪酸 (SCFA). 这种技术准确地绘制了SCFA分布图,有助于对肠道微生物群和炎症疾病的研究.

科学领域:

代谢学代谢学代谢学
质谱仪成像成像质谱仪成像
肠道微生物组研究研究

背景情况:

短链脂肪酸 (SCFA) 是关键的肠道微生物群代谢物,影响宿主炎症和代谢疾病.
精确地量化肠道组织中SCFA的空间含量,对于理解它们的生物学作用至关重要.
当前的方法在异质组织环境中可视化和量化SCFA面临挑战.

研究的目的:

开发和验证一种同位素编码的组织衍生方法,与 MALDI-MSI 结合用于空间 SCFA 分析.
为了提高SCFA成像在结肠组织的灵敏度和准确性.
为了研究SCFA的空间变化在肠球炎的小鼠模型.

主要方法:

开发一种使用TMPA和D3-TMPA进行SCFA标签的同位素编码衍生.
应用矩阵辅助激光消耗/电离质谱成像 (MALDI-MSI) 进行空间SCFA映射.
使用D3-TMPA标记的SCFA作为内部标准来纠正矩阵效应并改善量化.

主要成果:

在结肠组织中成功可视化了多种SCFA (乙,,黄油,,六,酸,酸) 的存在.
在衍生后,MALDI-MSI对SCFA检测的灵敏度和准确度显著提高.
来自实验诱导肠球炎的小鼠结肠组织中的空间SCFA变化的表征.

结论:

开发的同位素编码的MALDI-MSI方法为成像SCFA在组织中的空间表达提供了可靠的方法.
这一战略为SCFA在肠道微生物群功能和疾病发病过程中的作用提供了新的见解.
该方法使精确的空间量化成为可能,这对于研究SCFA参与炎症和代谢条件至关重要.

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