通过对大米 WRKY 转录因子的转化调节来提高植物免疫力
Chao Zheng1,2, Jie Zhou1, Xiaoya Yuan1,2
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Ministry of Agriculture Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, P. R. China.
通过控制 OsWRKY7 转录因子,提高了对细菌病原体的防治力. 这涉及通过替代翻译和稳定性调节其蛋白质水平,促进植物防御机制.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病理学 植物病理学
- 遗传学 遗传学 是一个
背景情况:
- WRKY转录因子是植物免疫力的关键调节者.
- 在转录后对WRKY基因功能的调节还不清楚.
- OsWRKY7是对抗Xanthomonas oryzae pv.米的基本免疫的积极调节者. 奥里扎 (Xoo) 的意思.
研究的目的:
- 为了研究大米免疫中OsWRKY7的转录后调节.
- 了解如何替代翻译和蛋白质稳定性控制 OsWRKY7 功能.
- 为了探索OsWRKY7在防治细菌病害的米防御中的作用.
主要方法:
- 使用替代启动对OsWRKY7转化产品的分析.
- 通过ubiquitin-proteasome系统研究OsWRKY7蛋白质的稳定性.
- 评估OsWRKY7在酵母和大米细胞中的转录活性.
- 评估 OsWRKY7 干扰对细菌炎症耐药性的影响.
主要成果:
- OsWRKY7通过替代启动产生了两个翻译产品.
- 全长的OsWRKY7被ubiquitin-proteasome系统降解,但在病原体处理时会积累.
- 替代的OsWRKY7产品是稳定的,具有转录活性.
- 这两种OsWRKY7形式的过度表达增强了对Xoo的抵抗力.
- 破坏主要的AUG编码子会增加稳定的替代形式并增强抗性.
结论:
- OsWRKY7的功能由替代翻译和蛋白质稳定性来调节.
- 这项规定对于协调植物生长和基底防御至关重要.
- 控制OsWRKY7的翻译启动为培养抗病大米提供了一种策略.
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