多功能阴离子高分支聚氨基糖化物,针对重度腹部创伤管理的多个介质
Yongqiang Xiao1,2,3, He Fang1, Yuefei Zhu2
1Department of Burn Surgery, the First Affiliated Hospital, Naval Medical University, Shanghai, 200433, P. R. China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|November 24, 2023
概括
新的生物材料,高分支聚氨基糖化物 (HPT和ss-HPT),有效地清除无细胞核酸 (cfNAs),以减少创伤后的炎症和凝血. 这种ss-HPT变种显示出有希望的有针对性的输送和生物降解,以改善创伤患者的治疗结果.
科学领域:
- 生物材料科学 生物材料科学
- 免疫学 免疫学 免疫学
- 创伤病理生理学
背景情况:
- 创伤会引发严重的并发症,如炎症,感染和DIC,原因是无细胞核酸 (cfNA) 作为损伤相关分子模式 (DAMP) 起作用.
- 基于生物材料的cfNA清理提供了一种潜在的治疗策略,以减轻创伤后的全身炎症和凝血异常.
研究的目的:
- 为了研究阴离子超分支聚氨基糖化物 (HPT) 和含二硫化物HPT (ss-HPT) 在清理cfNAs中的有效性.
- 评估它们在缓解创伤后炎症和高凝血方面的潜力.
主要方法:
- 来自托布拉米辛的HPT和ss-HPT聚合物的合成.
- 在体外评估cfNA清除,DAMP诱导的TLR激活,炎症性细胞因子分泌和高凝血.
- 在小鼠腹部创伤模型中进行体内评估,包括器官积累,保留和生物降解研究.
主要成果:
- 无论是HPT还是ss-HPT都有效地清除了cfNA,抑制了TLR激活,细胞因子释放和高凝血.
- 聚合物表现出由于托布拉米辛的抗菌活性.
- 在CLP模型中,HPT和ss-HPT调节了炎症,改善了治疗结果.
- 与HPT相比,ss-HPT在受伤器官中增加了积累,在健康小鼠中生物降解速度更快.
结论:
- 功能化ss-HPT是一种生物可降解的聚氨基糖化物,有效地清除cfNA并表现出治疗潜力.
- ss-HPT有望通过减少炎症和凝血并发症来改善创伤患者的治疗结果.
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