低密度中性粒细胞亚群的表型和氧化突发在常见的可变免疫缺陷患者中发生变化
Peter Slanina1,2, Julie Stichova1,2, Veronika Bosakova3,4
1Department of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Cytometry. Part B, Clinical cytometry
|November 24, 2023
概括
常见的可变免疫缺陷障碍 (CVID) 患者显示低密度中性粒细胞 (LDNs) 的增加. 在CVID患者中,成熟的LDNs降低了氧化爆发能力,可能会增加感染风险.
科学领域:
- 免疫学 免疫学 免疫学
- 血液学 血液学 血液学
- 细胞生物学 细胞生物学
背景情况:
- 常见的可变免疫缺陷障碍 (CVID) 是一种主要抗体缺乏症.
- 冠状病毒感染患者经常出现慢性粒细胞激活,包括增加低密度中性粒细胞 (LDNs).
- 免疫球蛋白替代疗法是CVID的标准治疗方法.
研究的目的:
- 调查CVID患者的LDN百分比和表面标记物表达.
- 在CVID中评估LDN的功能能力 (氧化爆发).
- 探索体外IVIg刺激对LDN的影响.
主要方法:
- 来自25名CVID患者和27名健康供体 (HD) 的全血被IVIg刺激.
- 用CD10和CD16表达 (mLDNs与iLDNs) 来量化和表征LDN群体.
- 用功能测定测量LDN的氧化爆发能力.
主要成果:
- 冠状病毒感染患者的相对和绝对LDN数量较高,成熟LDN (mLDN) 的比例更高.
- 与mLDNs相比,CVID和HD组在不成熟的LDNs (iLDNs) 中显示氧化爆发能力降低.
- 在CVID患者中,成熟的LDN显示氧化爆发能力降低,刺激后CD10表达较低,PD-L1表达较高.
结论:
- LDN的功能特征与它们的发育阶段有关.
- 在CVID患者的成熟LDN中,氧化爆发能力降低可能导致对细菌感染的易感性.
- 对CVID中LDN功能的进一步研究是有必要的.
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