相关实验视频
Updated: Jul 10, 2025
10:40
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
Published on: April 25, 2022
2.4K
通过DNA调节器的MiRNA响应CRISPR-Cas系统
1Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Biosensors
|November 24, 2023
概括
研究人员开发了一种由microRNAs (miRNAs) 调节的新型CRISPR激活系统. 这种简单的系统允许通过在miRNA结合时释放CRISPR相关蛋白9 (Cas9) 活性来控制基因编辑.
科学领域:
- 分子生物学分子生物学
- 生物技术是生物技术.
- 遗传学 遗传学 是一个
背景情况:
- 集群定期间隔的短平行体重复 (CRISPR) - 与CRISPR相关的蛋白9 (Cas9) 是一种多功能基因组编辑工具.
- 现有的调节CRISPR-Cas9活动的方法往往涉及复杂的工程.
- 对于CRISPR-Cas9技术,需要简单方便的监管系统.
研究的目的:
- 设计一个简单方便的CRISPR激活系统,由微RNAs (miRNAs) 调节.
- 为了创建一个可以被特定的miRNA激活的DNA调节器,从而控制CRISPR-Cas9活动.
主要方法:
- 设计了一种具有两个组成部分的DNA调节器:一种与Cas9-ribonucleoprotein (RNP) 复合体结合的抑制组件 (PAM和种子序列),以及一种miRNA识别组件.
- 该miRNA识别组件具有用于目标miRNA结合的脚DNA和用于miRNA互补性的部分双链DNA.
- 目标miRNAs结合后,调节器的结构被破坏,导致其与RNP复合体分离,并恢复CRISPR活性.
主要成果:
- 开发的系统成功地证明了miRNA激活的CRISPR-Cas9活动的调节.
- DNA调节器有效地抑制了Cas9-RNP复合物的活性,直到目标miRNA结合发生.
- 该系统通过简单的序列修改显示了对各种miRNA的应用潜力.
结论:
- 这项研究提供了一个通用且适应性强的平台,用于使用miRNA激活的CRISPR系统进行受控基因组编辑.
- 开发的系统提供了一种简单方便的方法来调节CRISPR-Cas9活动.
- 这种方法通过使miRNA对基因组编辑过程进行特定控制来促进精确的基因操纵.
相关概念视频
01:59CRISPR
51.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
51.8K
02:53CRISPR and crRNAs
17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
01:23RNA Interference
26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K
02:31Homologous Recombination
50.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.6K
02:02Cis-regulatory Sequences
9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
01:22MicroRNAs
21.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.3K