相关实验视频

Updated: Jul 10, 2025

A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction

A Semi-Quantitative Drug Affinity Responsive Target Stability DARTS assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

mTOR 抑制剂 Rapalink-1 阻止了内皮细胞中乙醇诱导的衰老

Huakang Zhou¹, Xuanchen Li¹, Majeed Rana²

¹Department of Neurosurgery, Medical Faculty, University Hospital Düsseldorf, Heinrich-Heine-University, Moorenstrasse 5, 40225 Düsseldorf, Germany.

|November 24, 2023

概括

拉帕林克-1是一种mTOR抑制剂,可以预防由乙醇和氧化应激引起的细胞衰老和血管炎症. 这种化合物通过抑制促炎途径和矩阵金属蛋白酶-2.2来保护心血管疾病.

科学领域:

心血管研究研究心血管研究
细胞生物学细胞生物学
衰老研究研究衰老研究

背景情况:

心血管风险因素,如乙醇和氧化应激诱导细胞衰老.
衰老细胞释放促炎分子和矩阵金属蛋白酶 (MMP),促进血管炎症和细胞外矩阵降解.
这些过程有助于心血管疾病的开始和进展.

研究的目的:

研究Rapalink-1,一种mTOR抑制剂对乙醇诱导的细胞衰老和相关的血管炎症的影响.
评估Rapalink-1对DNA损伤,衰老标志物和内皮细胞炎症通路的影响.

主要方法:

暴露于乙醇的内皮细胞被用Rapalink-1治疗.
评估了DNA损伤,衰老标记物 (P21,Lamin B1) 和DNA修复蛋白 (KU70).
分析了NF-κB,MAPKs (P38,ERK) 和mTOR通路蛋白的激活.
测量了炎症标记物的mRNA表达 (ICAM-1,E-选择素,MCP-1,IL-8) 和MMPs (MMP-2,TIMP-2).

主要成果:

拉帕林克-1 抑制了氧化应激诱导的DNA损伤和老化在暴露于乙醇的内皮细胞中.
它降低了P21表达,增加了KU70和Lamin B1表达,并抑制了NF-κB,MAPK和mTOR通路激活.
拉帕林克-1抑制了ICAM-1,E-selectin,MCP-1,IL-8,MMP-2和TIMP-2的mRNA表达,并降低了MMP-2蛋白水平.

结论:

拉帕林克-1有效地防止乙醇诱导的细胞衰老和血管炎症.
该化合物通过抑制促炎途径激活和减少炎性分子和MMP-2的表达而起作用.
拉帕林克-1显示出作为治疗与衰老相关的心血管疾病的治疗剂的潜力.

关键词:

这就是MAPKs.在 NFκ-BB 中.拉帕林克-1 连接器在 SASP 找 SASP 内皮细胞是内皮细胞.乙醇乙醇是一种在mTOROR中使用mTOR.衰老是一种老化.

更多相关视频

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

Published on: September 12, 2019

相关概念视频

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...