一个高通量,高制的人类初级上皮层气道器官芯片平台,用于SARS-CoV-2治疗查
Christine R Fisher1, Felix Mba Medie1, Rebeccah J Luu1
1Bioengineering Division, Draper, Cambridge, MA 02139, USA.
Cells
|November 24, 2023
概括
一个新的人体器官芯片平台PREDICT96-ALI成功模拟了SARS-CoV-2感染和病毒复制. 这种高通量系统证明了抗病毒疗效,有助于COVID-19的治疗发现.
科学领域:
- 病毒学 病毒学
- 器官在芯片上的技术
- 传染病建模传染病模型
背景情况:
- 随着COVID-19的流行,人们越来越需要有效的治疗方法.
- 有限的人类相关的临床前模型阻碍了对SARS-CoV-2的药物查.
- 对于本地病毒研究,通常需要高度封闭的设施.
研究的目的:
- 建立和验证用于SARS-CoV-2感染建模的高通量人体器官芯片平台.
- 在人类肺组织中调查SARS-CoV-2感染动力学.
- 用这个平台来评估抗病毒化合物的疗效.
主要方法:
- 利用PREDICT96-ALI平台与人类初级细胞模拟SARS-CoV-2感染.
- 在6天内使用免疫光,RT-qPCR和斑块检测评估病毒复制和感染动力学.
- 进行了Remdesivir和MPro61.1.的概念验证抗病毒研究.
主要成果:
- 在PREDICT96-ALI平台上展示了强大的SARS-CoV-2感染和复制.
- 从三个人类捐赠者的肺组织中表现出明显的感染动力学特征.
- 在平台上确认了雷梅西维尔和MPro61对SARS-CoV-2的抗病毒作用.
结论:
- PREDICT96-ALI平台为SARS-CoV-2研究提供了一个与人类生理学相关的体外系统.
- 这种高通量器官芯片模型对于推进COVID-19疾病建模至关重要.
- 该平台使新型抗病毒药物的有效治疗查成为可能.
关键词:
在 COVID-19 疫情中,这就是SARS-CoV-2病毒.这是一种抗病毒药物.冠状病毒冠状病毒的新冠病毒.皮质细胞是表皮细胞.微流体学 在微流体学方面器官模型器官模型器官模型流行病是一种流行病.呼吸道 呼吸道 呼吸道通过量通过量.更多相关视频
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