评估两种用于在尿样中直接检测CTX-M生产者的快速检测测试
Forrest Tang1, Chung-Ho Lee1, Xin Li2,3,4
1Department of Clinical Pathology, Kwong Wah Hospital, Hospital Authority, Hong Kong, China.
Antibiotics (Basel, Switzerland)
|November 24, 2023
概括
快速测试可以准确地检测尿液中的CTX-M产生肠道细菌. CTX-M MULTI侧流检测显示出比ESBL NP测试更高的灵敏度用于诊断尿路感染.
科学领域:
- 临床微生物学 临床微生物学
- 传染性疾病 传染性疾病
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 产生扩展谱β-乳糖酶 (ESBL) 的肠细菌感染是全球日益严重的健康问题.
- CTX-M酶是肠杆菌中ESBL产生的主要原因.
- 快速准确地检测这些耐药生物对于有效的患者管理和感染控制至关重要.
研究的目的:
- 评估NG-Test® CTX-M MULTI侧流量测试 (CTX-M LFA) 和快速ESBL NP®测试 (ESBL NP测试) 的性能.
- 评估它们在直接从中流尿样 (MSU) 中快速检测CTX-M产生的肠道细菌方面的有用性.
主要方法:
- 总共有277个临床MSU样本被前性和后性测试.
- 性能与表型ESBL检测和分子测试 (blaCTX-M基因的PCR) 作为参考标准进行了比较.
- 包括对样本进行测试,其中的格拉姆染料显示了格拉姆阴性细菌.
主要成果:
- 总体而言,在277个样本中,67个样本对ESBL产生肠道细菌的培养呈阳性.
- CTX-M LFA 显示了 100% 的灵敏度 (95% CI 94.6-100%) 和 99.1% 的特异性 (95% CI 96.6-99.9%).
- ESBL NP测试显示了86.6%的灵敏度 (95% CI 76.0-93.7%) 和100%的特异性 (95% CI 98.2-100%),有三个不可解释的结果.
结论:
- 无论是CTX-M LFA测试还是ESBL NP测试,都能快速检测尿液中的CTX-M产生的肠道细菌.
- 在本次评估中,CTX-M LFA 显示出更高的灵敏度.
- 这些快速测试可能会提高针对尿路感染的抗菌药物管理.
关键词:
在CTX-M中使用.它们是Enterobacteriaceae的细菌.贝塔 - 乳糖酶酶的存在颜色测量试验的测试方法扩展光谱β-lactamases是一种扩展光谱β-lactamases.免疫染色学测定检测方法尿路感染 尿路感染更多相关视频
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