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传统文化与下一代对疑似骨科感染的测序:从参考中心获得的经验

Sara Giordana Rimoldi¹, Davide Brioschi², Daniele Curreli¹

¹Laboratory of Clinical Microbiology, Virology and Bioemergencies, ASST Fatebenefratelli Sacco, "L. Sacco" University Hospital, Via Giovanni Battista Grassi n. 74, 20157 Milan, Italy.

Antibiotics (Basel, Switzerland)

|November 24, 2023

概括

下一代测序 (NGS) 显示出对诊断骨科感染的希望,检测出比传统培养更多的细菌,包括难以生长的类型. 需要进一步的研究来区分病原体和污染物.

科学领域:

整形外科整形外科整形外科
传染性疾病传染性疾病
分子诊断学分子诊断学

背景情况:

骨科感染带来了诊断挑战,特别是在培养负结果的情况下.
传统的培养方法难以检测某些细菌,影响治疗决策.
下一代测序 (NGS) 提供了在骨科感染中改善病原体检测的潜力.

研究的目的:

为了评估NGS与传统培养相比对诊断骨科感染的现实世界的影响.
评估NGS和培养方法之间的一致率和不一致率.
确定NGS在确定骨科感染中难以生长的微生物的有用性.

主要方法:

一项前性研究包括49名怀疑有骨科感染的患者接受手术.
结合传统的细菌培养与元基因16S核糖体RNA测序 (NGS).
应用了1000个计数的百分比频率读取切断对NGS来最大限度地减少假阳性.

主要成果:

在73%的病例中,NGS检测到细菌,而传统培养则为59%.
NGS和培养之间的一致性为61%.
在19个不一致的病例中,NGS在11个病例中确定了培养过程中遗漏的病原体,其中包括难以生长的细菌.

结论:

与标准培养相比,NGS表现出优异的细菌检测能力,特别是缓慢生长的无氧类型.
NGS可以作为骨科感染的有价值的辅助诊断工具.
需要进一步的研究来完善NGS解释,以区分真正的病原体和污染物.

关键词:

骨感染骨感染这是下一代测序.假肢关节感染假肢关节感染

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